Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23133 | 3' | -58 | NC_005178.1 | + | 1284 | 0.69 | 0.281867 |
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Target: 5'- gGCAGCGUCcgaaucGCUCAGaGaCGCugCUGAc- -3' miRNA: 3'- -CGUCGCAG------UGGGUC-C-GCGugGACUac -5' |
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| 23133 | 3' | -58 | NC_005178.1 | + | 22871 | 0.7 | 0.241123 |
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Target: 5'- gGCGGuCGUgGCCCAGGCuauCGCC-GAUGc -3' miRNA: 3'- -CGUC-GCAgUGGGUCCGc--GUGGaCUAC- -5' |
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| 23133 | 3' | -58 | NC_005178.1 | + | 8819 | 0.71 | 0.222658 |
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Target: 5'- gGCaAGaaaGUCGCCCGGcGCGUGCCgGGUGu -3' miRNA: 3'- -CG-UCg--CAGUGGGUC-CGCGUGGaCUAC- -5' |
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| 23133 | 3' | -58 | NC_005178.1 | + | 10583 | 0.71 | 0.216776 |
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Target: 5'- gGUAGCG--AUCCAGGCGCGCCUucUGc -3' miRNA: 3'- -CGUCGCagUGGGUCCGCGUGGAcuAC- -5' |
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| 23133 | 3' | -58 | NC_005178.1 | + | 25727 | 0.71 | 0.211027 |
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Target: 5'- aCAGCc---UCCAGGCGCGCCUGAa- -3' miRNA: 3'- cGUCGcaguGGGUCCGCGUGGACUac -5' |
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| 23133 | 3' | -58 | NC_005178.1 | + | 11821 | 0.72 | 0.18933 |
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Target: 5'- gGCAGC---AUUCAGGCGCGCCUGGa- -3' miRNA: 3'- -CGUCGcagUGGGUCCGCGUGGACUac -5' |
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| 23133 | 3' | -58 | NC_005178.1 | + | 14083 | 0.74 | 0.128173 |
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Target: 5'- aGCAGCGggccggcuUCGCgCCGGGCGC-CCUGGc- -3' miRNA: 3'- -CGUCGC--------AGUG-GGUCCGCGuGGACUac -5' |
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| 23133 | 3' | -58 | NC_005178.1 | + | 8592 | 0.75 | 0.121093 |
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Target: 5'- cGCAGgGUCACCU-GGCGCACggcGAUGg -3' miRNA: 3'- -CGUCgCAGUGGGuCCGCGUGga-CUAC- -5' |
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| 23133 | 3' | -58 | NC_005178.1 | + | 27236 | 0.77 | 0.085736 |
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Target: 5'- cCAGCGUCACCgAGGCGaagaacCGCCUGGa- -3' miRNA: 3'- cGUCGCAGUGGgUCCGC------GUGGACUac -5' |
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| 23133 | 3' | -58 | NC_005178.1 | + | 21145 | 1.1 | 0.000247 |
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Target: 5'- gGCAGCGUCACCCAGGCGCACCUGAUGa -3' miRNA: 3'- -CGUCGCAGUGGGUCCGCGUGGACUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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