Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23133 | 3' | -58 | NC_005178.1 | + | 6389 | 0.67 | 0.401219 |
Target: 5'- aGgGGCGUCugCgCucgauugucgcggcgAGGCGCGCCgcGAUGa -3' miRNA: 3'- -CgUCGCAGugG-G---------------UCCGCGUGGa-CUAC- -5' |
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23133 | 3' | -58 | NC_005178.1 | + | 26408 | 0.66 | 0.416325 |
Target: 5'- gGCGGCG--GCCaGGGCGCccGCgCUGAUGu -3' miRNA: 3'- -CGUCGCagUGGgUCCGCG--UG-GACUAC- -5' |
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23133 | 3' | -58 | NC_005178.1 | + | 20393 | 0.66 | 0.425941 |
Target: 5'- cCGGCccuggaCGCCCAGGCGaccaACCUGGc- -3' miRNA: 3'- cGUCGca----GUGGGUCCGCg---UGGACUac -5' |
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23133 | 3' | -58 | NC_005178.1 | + | 6486 | 0.66 | 0.425941 |
Target: 5'- gGCAGCaaaACCCAgcggcGGCGaCGCCUGGc- -3' miRNA: 3'- -CGUCGcagUGGGU-----CCGC-GUGGACUac -5' |
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23133 | 3' | -58 | NC_005178.1 | + | 364 | 0.66 | 0.425941 |
Target: 5'- -uGGCGUCAaacucggccUCCAGGCGCAgCgGGUc -3' miRNA: 3'- cgUCGCAGU---------GGGUCCGCGUgGaCUAc -5' |
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23133 | 3' | -58 | NC_005178.1 | + | 24805 | 0.66 | 0.445562 |
Target: 5'- -gGGCGUCGCggguuuccuUCGGGUGCGCCaUGGa- -3' miRNA: 3'- cgUCGCAGUG---------GGUCCGCGUGG-ACUac -5' |
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23133 | 3' | -58 | NC_005178.1 | + | 16395 | 0.66 | 0.445562 |
Target: 5'- gGCGG-GUUucaUCAGGUGCGCCUGGg- -3' miRNA: 3'- -CGUCgCAGug-GGUCCGCGUGGACUac -5' |
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23133 | 3' | -58 | NC_005178.1 | + | 22767 | 0.66 | 0.45556 |
Target: 5'- aCAGUGagcuauugCACCCAGGCcgACCUGGUc -3' miRNA: 3'- cGUCGCa-------GUGGGUCCGcgUGGACUAc -5' |
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23133 | 3' | -58 | NC_005178.1 | + | 12135 | 0.66 | 0.45556 |
Target: 5'- gGCGGCGaccaUCGCCUugauccGGGC-CGCCUGcUGg -3' miRNA: 3'- -CGUCGC----AGUGGG------UCCGcGUGGACuAC- -5' |
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23133 | 3' | -58 | NC_005178.1 | + | 26955 | 0.66 | 0.465678 |
Target: 5'- -gGGCGagaaggCGCagaAGGCGCGCCUGGa- -3' miRNA: 3'- cgUCGCa-----GUGgg-UCCGCGUGGACUac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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