Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23144 | 3' | -54.1 | NC_005178.1 | + | 31595 | 0.7 | 0.458278 |
Target: 5'- cCGGCCUGGAc---CUGguccgCCCCGuCCa -3' miRNA: 3'- -GUCGGACCUucaaGACaa---GGGGCuGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 31215 | 0.7 | 0.458278 |
Target: 5'- -cGCCUGGAcGUUCg---CCCCgGACUg -3' miRNA: 3'- guCGGACCUuCAAGacaaGGGG-CUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 30443 | 0.67 | 0.622894 |
Target: 5'- cCGGCCgcUGGAGGUacucgCUGca-CUCGACCg -3' miRNA: 3'- -GUCGG--ACCUUCAa----GACaagGGGCUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 26916 | 0.66 | 0.657035 |
Target: 5'- cCGGCCgGGgcGU--UGcgCCCCGGCUu -3' miRNA: 3'- -GUCGGaCCuuCAagACaaGGGGCUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 24665 | 1.13 | 0.000425 |
Target: 5'- aCAGCCUGGAAGUUCUGUUCCCCGACCg -3' miRNA: 3'- -GUCGGACCUUCAAGACAAGGGGCUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 23255 | 0.69 | 0.511106 |
Target: 5'- cCGGCCUucaGGAcGUUUcgGgcgUCCCCGAUCu -3' miRNA: 3'- -GUCGGA---CCUuCAAGa-Ca--AGGGGCUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 21955 | 0.75 | 0.2151 |
Target: 5'- cUAGCCUGGAGGUgcccCUGcucggCCUCGAUCa -3' miRNA: 3'- -GUCGGACCUUCAa---GACaa---GGGGCUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 19547 | 0.66 | 0.690952 |
Target: 5'- cCAGCCUGGAg---CUGggcaCCCUGGgCa -3' miRNA: 3'- -GUCGGACCUucaaGACaa--GGGGCUgG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 15518 | 0.67 | 0.586567 |
Target: 5'- cCGGUCUGGccaaaGAGUUCgccaaccaCCCGGCCa -3' miRNA: 3'- -GUCGGACC-----UUCAAGacaag---GGGCUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 12229 | 0.72 | 0.327955 |
Target: 5'- gCGGCUUGGAucgagUCggacaGUUCCUCGACCu -3' miRNA: 3'- -GUCGGACCUuca--AGa----CAAGGGGCUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 8793 | 0.7 | 0.448061 |
Target: 5'- aCGGCCUGGcGGUUCUuGUUCagCCaGACg -3' miRNA: 3'- -GUCGGACCuUCAAGA-CAAGg-GG-CUGg -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 4304 | 0.66 | 0.657035 |
Target: 5'- -uGcCCUGGAaauGGaugcgccgCUGUccaUCCCCGGCCu -3' miRNA: 3'- guC-GGACCU---UCaa------GACA---AGGGGCUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 3008 | 0.69 | 0.479076 |
Target: 5'- gCAGCCUGG-AGcgCU--UCCUgGACCa -3' miRNA: 3'- -GUCGGACCuUCaaGAcaAGGGgCUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 2196 | 0.67 | 0.63428 |
Target: 5'- gGGCCUGGAGcgUCacg-CCCUGGCUg -3' miRNA: 3'- gUCGGACCUUcaAGacaaGGGGCUGG- -5' |
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23144 | 3' | -54.1 | NC_005178.1 | + | 1505 | 0.73 | 0.311728 |
Target: 5'- gGGCCaaGcGGUUCUGguucgCCCCGGCCc -3' miRNA: 3'- gUCGGacCuUCAAGACaa---GGGGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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