Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23154 | 3' | -54.2 | NC_005178.1 | + | 13889 | 0.7 | 0.393816 |
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Target: 5'- cCGC-CCGGCGugAACGGGccaGCGCg- -3' miRNA: 3'- -GCGaGGUCGUugUUGUCCua-CGCGac -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 27497 | 0.71 | 0.348111 |
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Target: 5'- aGCUCCAGgGGCGAUGG---GCGCUGg -3' miRNA: 3'- gCGAGGUCgUUGUUGUCcuaCGCGAC- -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 7311 | 0.71 | 0.339437 |
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Target: 5'- uGCUCCAGC-GCAugGGGGUcCGCc- -3' miRNA: 3'- gCGAGGUCGuUGUugUCCUAcGCGac -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 20286 | 0.72 | 0.314363 |
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Target: 5'- gGcCUCCAGgauCGGCcGCAGGuUGCGCUGg -3' miRNA: 3'- gC-GAGGUC---GUUGuUGUCCuACGCGAC- -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 28915 | 0.72 | 0.290715 |
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Target: 5'- -cCUCCAGCAGCcgAACGGGAUcGUGCg- -3' miRNA: 3'- gcGAGGUCGUUG--UUGUCCUA-CGCGac -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 25802 | 0.75 | 0.198583 |
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Target: 5'- aGCUCCAGgAGCAACGGGAccGC-CUGc -3' miRNA: 3'- gCGAGGUCgUUGUUGUCCUa-CGcGAC- -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 14301 | 0.75 | 0.182483 |
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Target: 5'- gGCUCCGGCAA--GC-GGAUGCGCg- -3' miRNA: 3'- gCGAGGUCGUUguUGuCCUACGCGac -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 25436 | 0.83 | 0.057534 |
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Target: 5'- cCGcCUCCcuGCAACAGCGGGAaGCGCUGg -3' miRNA: 3'- -GC-GAGGu-CGUUGUUGUCCUaCGCGAC- -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 27613 | 1.09 | 0.000607 |
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Target: 5'- gCGCUCCAGCAACAACAGGAUGCGCUGg -3' miRNA: 3'- -GCGAGGUCGUUGUUGUCCUACGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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