Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23154 | 3' | -54.2 | NC_005178.1 | + | 28014 | 0.68 | 0.495957 |
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Target: 5'- uCGCUCguuGGCGuucccGCGcCAGGcgGCGCUGg -3' miRNA: 3'- -GCGAGg--UCGU-----UGUuGUCCuaCGCGAC- -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 28915 | 0.72 | 0.290715 |
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Target: 5'- -cCUCCAGCAGCcgAACGGGAUcGUGCg- -3' miRNA: 3'- gcGAGGUCGUUG--UUGUCCUA-CGCGac -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 29137 | 0.66 | 0.59694 |
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Target: 5'- uGCaUCAGCcuggggUAACAGGAaGCGCUGa -3' miRNA: 3'- gCGaGGUCGuu----GUUGUCCUaCGCGAC- -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 30225 | 0.69 | 0.443234 |
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Target: 5'- gGCUCCAgGCGAUGAaggcGGAcccccaUGCGCUGg -3' miRNA: 3'- gCGAGGU-CGUUGUUgu--CCU------ACGCGAC- -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 30401 | 0.68 | 0.521151 |
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Target: 5'- aCGCuuUCCAGCAgcgcaucGCGACGGGGUuguucagcggcgccgGcCGCUGg -3' miRNA: 3'- -GCG--AGGUCGU-------UGUUGUCCUA---------------C-GCGAC- -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 31451 | 0.66 | 0.654595 |
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Target: 5'- uGCUCCAGgAACcGCuGGcgGCcggcucGCUGa -3' miRNA: 3'- gCGAGGUCgUUGuUGuCCuaCG------CGAC- -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 33537 | 0.68 | 0.517835 |
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Target: 5'- -aCUCCAguucGCAGCGGCGGacGGUGCGCa- -3' miRNA: 3'- gcGAGGU----CGUUGUUGUC--CUACGCGac -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 34409 | 0.66 | 0.619979 |
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Target: 5'- -uCUCCAGCAgugcauggGCAGCGcGGGcgcUGCGCUu -3' miRNA: 3'- gcGAGGUCGU--------UGUUGU-CCU---ACGCGAc -5' |
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| 23154 | 3' | -54.2 | NC_005178.1 | + | 34588 | 0.66 | 0.59694 |
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Target: 5'- gCGCUCgGGCGGCcGCgAGGAU-CGCg- -3' miRNA: 3'- -GCGAGgUCGUUGuUG-UCCUAcGCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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