Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23163 | 3' | -58.9 | NC_005178.1 | + | 9034 | 0.74 | 0.119242 |
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Target: 5'- aUGUAGCGCCGCCcauCCu-CGuUGGUUGAu -3' miRNA: 3'- -ACAUCGCGGCGGu--GGcuGC-ACCAGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 7091 | 0.69 | 0.260564 |
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Target: 5'- -aUGGCGCaCGgCACC-ACGuUGGUCGAg -3' miRNA: 3'- acAUCGCG-GCgGUGGcUGC-ACCAGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 6736 | 0.68 | 0.31068 |
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Target: 5'- ---uGCGCCGCguagaCGCCGGCccaGGUCGAg -3' miRNA: 3'- acauCGCGGCG-----GUGGCUGca-CCAGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 6504 | 0.66 | 0.451155 |
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Target: 5'- gGUcacCGCCGCCACCGuGCGcaugcGGUUGGc -3' miRNA: 3'- aCAuc-GCGGCGGUGGC-UGCa----CCAGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 5991 | 0.69 | 0.260564 |
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Target: 5'- --gGGCGCUGCCccagGCUGGCGUG-UCGGa -3' miRNA: 3'- acaUCGCGGCGG----UGGCUGCACcAGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 4988 | 0.66 | 0.441381 |
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Target: 5'- ----cUGUCGCCGCUGGCcaGGUCGAg -3' miRNA: 3'- acaucGCGGCGGUGGCUGcaCCAGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 3072 | 0.67 | 0.385391 |
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Target: 5'- -uUGGCGCUGCgCcCCGuCGaucUGGUCGAu -3' miRNA: 3'- acAUCGCGGCG-GuGGCuGC---ACCAGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 2515 | 0.73 | 0.153059 |
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Target: 5'- ---cGCGCCagaGCUGCCGACGcGGUCGGu -3' miRNA: 3'- acauCGCGG---CGGUGGCUGCaCCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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