Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23163 | 3' | -58.9 | NC_005178.1 | + | 13116 | 0.71 | 0.19535 |
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Target: 5'- cGgcGCGCCGCCuuCGGCcaGGUUGAu -3' miRNA: 3'- aCauCGCGGCGGugGCUGcaCCAGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 34836 | 0.71 | 0.19535 |
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Target: 5'- cGUGGCGCCGCCACCacCG-GGcUGGa -3' miRNA: 3'- aCAUCGCGGCGGUGGcuGCaCCaGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 26461 | 0.72 | 0.170724 |
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Target: 5'- --cGGCGCUGCCgGCCGAgGUGGaugCGGg -3' miRNA: 3'- acaUCGCGGCGG-UGGCUgCACCa--GCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 2515 | 0.73 | 0.153059 |
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Target: 5'- ---cGCGCCagaGCUGCCGACGcGGUCGGu -3' miRNA: 3'- acauCGCGG---CGGUGGCUGCaCCAGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 25901 | 0.73 | 0.148909 |
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Target: 5'- --cGGCGCagaUCACCGACGUGGUCa- -3' miRNA: 3'- acaUCGCGgc-GGUGGCUGCACCAGcu -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 21183 | 0.74 | 0.126098 |
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Target: 5'- cGUAgGCGCCGCCGcCCGACucgcUGGUCu- -3' miRNA: 3'- aCAU-CGCGGCGGU-GGCUGc---ACCAGcu -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 9034 | 0.74 | 0.119242 |
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Target: 5'- aUGUAGCGCCGCCcauCCu-CGuUGGUUGAu -3' miRNA: 3'- -ACAUCGCGGCGGu--GGcuGC-ACCAGCU- -5' |
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| 23163 | 3' | -58.9 | NC_005178.1 | + | 32288 | 1.08 | 0.000314 |
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Target: 5'- cUGUAGCGCCGCCACCGACGUGGUCGAg -3' miRNA: 3'- -ACAUCGCGGCGGUGGCUGCACCAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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