Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23185 | 3' | -56.9 | NC_005259.1 | + | 35636 | 0.66 | 0.682819 |
Target: 5'- cGGGCC--GCCG-CcgCUGCCGcuGGCGa -3' miRNA: 3'- aCCUGGaaCGGCuGaaGACGGC--UCGC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 4741 | 0.66 | 0.682819 |
Target: 5'- aGGACCggcucagGCCGGuCccUCggGCCGGGCu -3' miRNA: 3'- aCCUGGaa-----CGGCU-Ga-AGa-CGGCUCGc -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 45115 | 0.66 | 0.650718 |
Target: 5'- cGGGCCgagaguggUGCCGACgaggucggcaaUCUGCUGAucGCu -3' miRNA: 3'- aCCUGGa-------ACGGCUGa----------AGACGGCU--CGc -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 49991 | 0.67 | 0.639973 |
Target: 5'- cGGAUCgcgUGCgGACggCUGCCcucgugcacGGGCGc -3' miRNA: 3'- aCCUGGa--ACGgCUGaaGACGG---------CUCGC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 3072 | 0.67 | 0.639973 |
Target: 5'- cGGGCCgagGCCGACaagagaGCCGcgcAGCGa -3' miRNA: 3'- aCCUGGaa-CGGCUGaaga--CGGC---UCGC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 17505 | 0.67 | 0.639973 |
Target: 5'- gGcGACCUUGCCGAU-----CCGAGCu -3' miRNA: 3'- aC-CUGGAACGGCUGaagacGGCUCGc -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 39052 | 0.67 | 0.62922 |
Target: 5'- gGGAUgUUGgCGAUgu-UGCCGAGCu -3' miRNA: 3'- aCCUGgAACgGCUGaagACGGCUCGc -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 7856 | 0.67 | 0.62922 |
Target: 5'- cGGACCgcGCCGACccCggugGCCcGAGUGc -3' miRNA: 3'- aCCUGGaaCGGCUGaaGa---CGG-CUCGC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 58571 | 0.67 | 0.61847 |
Target: 5'- uUGGGCCggauCgCGGCgcggCUGCCGGGCc -3' miRNA: 3'- -ACCUGGaac-G-GCUGaa--GACGGCUCGc -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 54728 | 0.67 | 0.607731 |
Target: 5'- aGGACauaGCCGAgg-CUGCCGAcaGCGa -3' miRNA: 3'- aCCUGgaaCGGCUgaaGACGGCU--CGC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 13224 | 0.67 | 0.592732 |
Target: 5'- gUGGGCCUcGaCCGGCcaccgugaggGCCGAGCa -3' miRNA: 3'- -ACCUGGAaC-GGCUGaaga------CGGCUCGc -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 8306 | 0.68 | 0.585255 |
Target: 5'- cGGaaacgccGCCUUGUCGGag-CUGCCGAcgGCGa -3' miRNA: 3'- aCC-------UGGAACGGCUgaaGACGGCU--CGC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 26620 | 0.68 | 0.565059 |
Target: 5'- cGGACCgguaGCCGAUaUCgaugGCCuGGCGu -3' miRNA: 3'- aCCUGGaa--CGGCUGaAGa---CGGcUCGC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 43839 | 0.68 | 0.554502 |
Target: 5'- aGGGCC--GCCG-CUcgccaUGCCGAGCGc -3' miRNA: 3'- aCCUGGaaCGGCuGAag---ACGGCUCGC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 45955 | 0.69 | 0.49262 |
Target: 5'- aGcACgUUGCCGACgu-UGCCGAGCu -3' miRNA: 3'- aCcUGgAACGGCUGaagACGGCUCGc -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 60258 | 0.7 | 0.443583 |
Target: 5'- gGGugCaagGCUGGCcaccagcgCUGCCGGGCGg -3' miRNA: 3'- aCCugGaa-CGGCUGaa------GACGGCUCGC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 23471 | 0.71 | 0.41643 |
Target: 5'- gGGGCCUcggcauugucggUGCCGACgccaugaccaagGCCGAGCu -3' miRNA: 3'- aCCUGGA------------ACGGCUGaaga--------CGGCUCGc -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 68851 | 0.71 | 0.415512 |
Target: 5'- gUGGGCCaucaUGUCGACUUgCccgcGCCGGGCGc -3' miRNA: 3'- -ACCUGGa---ACGGCUGAA-Ga---CGGCUCGC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 64428 | 0.71 | 0.397413 |
Target: 5'- gGGAUC--GCCGACgagCUGCCGGGgCGc -3' miRNA: 3'- aCCUGGaaCGGCUGaa-GACGGCUC-GC- -5' |
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23185 | 3' | -56.9 | NC_005259.1 | + | 27817 | 0.72 | 0.346258 |
Target: 5'- cGaGACCUgGCuCGACUUCUGCgCGAGg- -3' miRNA: 3'- aC-CUGGAaCG-GCUGAAGACG-GCUCgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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