Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23186 | 3' | -56.9 | NC_005259.1 | + | 61499 | 0.66 | 0.747122 |
Target: 5'- aCCguugaggCGCUCgGUGugccggucgaccuCGUCGGGAauuuccucgucUCGGGCg -3' miRNA: 3'- -GGa------GCGGGgUAU-------------GUAGCCCU-----------AGCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 158 | 0.66 | 0.727966 |
Target: 5'- gCUCGCCCgg-----CGGGGUCcGGCa -3' miRNA: 3'- gGAGCGGGguauguaGCCCUAGcCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 58652 | 0.66 | 0.726948 |
Target: 5'- cCCUCgcgguaGCCCgGUGCAcCGGGGUgcagcuuccaucgCGGGg -3' miRNA: 3'- -GGAG------CGGGgUAUGUaGCCCUA-------------GCCCg -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 63210 | 0.66 | 0.707459 |
Target: 5'- gCCUcgacCGCCUCGUcagACAgaUCGGGGUCGcuGGUc -3' miRNA: 3'- -GGA----GCGGGGUA---UGU--AGCCCUAGC--CCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 67363 | 0.66 | 0.703322 |
Target: 5'- aCCUCGCgCCGgaucuuggggaaauUACGcuuggccUCGGGGgugucaCGGGCc -3' miRNA: 3'- -GGAGCGgGGU--------------AUGU-------AGCCCUa-----GCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 62683 | 0.67 | 0.686679 |
Target: 5'- aCCUCGUCCUcgaGUGCGgugucccaucCGGcGAUCaGGCg -3' miRNA: 3'- -GGAGCGGGG---UAUGUa---------GCC-CUAGcCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 64211 | 0.67 | 0.686679 |
Target: 5'- aCCUCGCCggucggcagCCcgGUAUCGGGGUCGauaGCc -3' miRNA: 3'- -GGAGCGG---------GGuaUGUAGCCCUAGCc--CG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 65562 | 0.67 | 0.686679 |
Target: 5'- -aUCGgCCCAUagACAccgaGGGA-CGGGCg -3' miRNA: 3'- ggAGCgGGGUA--UGUag--CCCUaGCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 55612 | 0.67 | 0.676213 |
Target: 5'- gUCUCGCgCgCC-UugGcCGGGAUCaGGGCc -3' miRNA: 3'- -GGAGCG-G-GGuAugUaGCCCUAG-CCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 11226 | 0.67 | 0.665708 |
Target: 5'- --aCGCCgCAguccUCGGGcUCGGGCa -3' miRNA: 3'- ggaGCGGgGUauguAGCCCuAGCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 61062 | 0.67 | 0.65939 |
Target: 5'- aCCUUGUgCCGgGCAUCGGcAuaggcgucgucggccUCGGGCu -3' miRNA: 3'- -GGAGCGgGGUaUGUAGCCcU---------------AGCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 23552 | 0.67 | 0.655173 |
Target: 5'- gCCUCGaCCgCA-ACAUCacaaGGAUCGaGGCg -3' miRNA: 3'- -GGAGC-GGgGUaUGUAGc---CCUAGC-CCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 61391 | 0.67 | 0.64462 |
Target: 5'- --aCGCCCgAUGcCGUCGGuGuccucgCGGGCa -3' miRNA: 3'- ggaGCGGGgUAU-GUAGCC-Cua----GCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 32558 | 0.67 | 0.64462 |
Target: 5'- --cCGCCCCGgcgaGCG-CGGGGauagcgUUGGGCa -3' miRNA: 3'- ggaGCGGGGUa---UGUaGCCCU------AGCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 18107 | 0.68 | 0.635112 |
Target: 5'- gCCgUCGCgCUCAUcgacuacgagggcaACAUCGGcaaggccauggacgaGAUCGGGCu -3' miRNA: 3'- -GG-AGCG-GGGUA--------------UGUAGCC---------------CUAGCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 11416 | 0.68 | 0.634055 |
Target: 5'- gCUCGUggCCgGUGCAaCGGGGaugcUUGGGCc -3' miRNA: 3'- gGAGCG--GGgUAUGUaGCCCU----AGCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 52252 | 0.68 | 0.634055 |
Target: 5'- cCCg-GCCCCucuCAggGGGGcCGGGCa -3' miRNA: 3'- -GGagCGGGGuauGUagCCCUaGCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 34884 | 0.68 | 0.62349 |
Target: 5'- aCCggggUGCCUCGgGCAggaCGGGccgcUCGGGCa -3' miRNA: 3'- -GGa---GCGGGGUaUGUa--GCCCu---AGCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 28858 | 0.68 | 0.62349 |
Target: 5'- --cCGCCCCAccgagggACGUUGGGc-CGGGUa -3' miRNA: 3'- ggaGCGGGGUa------UGUAGCCCuaGCCCG- -5' |
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23186 | 3' | -56.9 | NC_005259.1 | + | 57204 | 0.68 | 0.591867 |
Target: 5'- aCCUCGaCgCCGUugAaaaaccgguugUCGGGGUCGGa- -3' miRNA: 3'- -GGAGC-GgGGUAugU-----------AGCCCUAGCCcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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