Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23188 | 3' | -58.4 | NC_005259.1 | + | 18849 | 0.69 | 0.421174 |
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Target: 5'- uGGCGAUCcgGCGCGUGaGCgaGCGGuggaaugcCCGCa -3' miRNA: 3'- gUCGCUAGa-CGCGCAC-CG--UGUC--------GGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 10082 | 0.69 | 0.411972 |
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Target: 5'- uCGGCGGUCUugucgucggcggGCGCgGUGGCAgcguCGGCCu- -3' miRNA: 3'- -GUCGCUAGA------------CGCG-CACCGU----GUCGGug -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 1031 | 0.69 | 0.393947 |
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Target: 5'- gUAGCGcgCgcaGCgGCGUGGCcACcGCCGCg -3' miRNA: 3'- -GUCGCuaGa--CG-CGCACCG-UGuCGGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 38563 | 0.7 | 0.335024 |
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Target: 5'- -cGCGAUgaGC-CGUGGCuguuCAGCUACg -3' miRNA: 3'- guCGCUAgaCGcGCACCGu---GUCGGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 1873 | 0.7 | 0.326372 |
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Target: 5'- -uGCGGUC-GUGCGUGaGCACcgcugugAGCCGCu -3' miRNA: 3'- guCGCUAGaCGCGCAC-CGUG-------UCGGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 7250 | 0.71 | 0.289856 |
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Target: 5'- cCGGCGAgaUCUGUGCGgcGGCAaucaccGCCGCa -3' miRNA: 3'- -GUCGCU--AGACGCGCa-CCGUgu----CGGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 40990 | 0.71 | 0.289856 |
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Target: 5'- gGGCGGggucgccggggUUGCGCGcguUGGC-CAGCCACg -3' miRNA: 3'- gUCGCUa----------GACGCGC---ACCGuGUCGGUG- -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 32451 | 0.72 | 0.249604 |
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Target: 5'- gGGUGAUCUGCgGUGUGGgAuucCAGCCAa -3' miRNA: 3'- gUCGCUAGACG-CGCACCgU---GUCGGUg -5' |
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| 23188 | 3' | -58.4 | NC_005259.1 | + | 2638 | 1.09 | 0.000515 |
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Target: 5'- gCAGCGAUCUGCGCGUGGCACAGCCACg -3' miRNA: 3'- -GUCGCUAGACGCGCACCGUGUCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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