Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23189 | 5' | -57.9 | NC_005259.1 | + | 25971 | 0.66 | 0.619602 |
Target: 5'- gAUGC-CGcCGAGCAGGCCGcCGaGCa -3' miRNA: 3'- aUGCGcGCaGCUCGUCCGGUaGUcUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 27869 | 0.66 | 0.619602 |
Target: 5'- gUGCcCGCGUCGcGCA-GCC-UCAGGCc -3' miRNA: 3'- -AUGcGCGCAGCuCGUcCGGuAGUCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 5149 | 0.66 | 0.608823 |
Target: 5'- --aGCGCGUaccggCGAGguGGCCggCuGGCc -3' miRNA: 3'- augCGCGCA-----GCUCguCCGGuaGuCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 38128 | 0.66 | 0.608823 |
Target: 5'- -uCGC-CGUCGAGCaAGGUC-UCAuGACg -3' miRNA: 3'- auGCGcGCAGCUCG-UCCGGuAGU-CUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 33383 | 0.66 | 0.598064 |
Target: 5'- cACGCuGCGUCGAGUAGu---UCGGGCc -3' miRNA: 3'- aUGCG-CGCAGCUCGUCcgguAGUCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 9883 | 0.66 | 0.587333 |
Target: 5'- cACGCGCGgcaUCGAGCAGaG-CGUCGaGCu -3' miRNA: 3'- aUGCGCGC---AGCUCGUC-CgGUAGUcUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 10061 | 0.66 | 0.586262 |
Target: 5'- gGCGCgguggcaGCGUCG-GCcuuGGCCAgcaggUCGGGCa -3' miRNA: 3'- aUGCG-------CGCAGCuCGu--CCGGU-----AGUCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 34113 | 0.67 | 0.565987 |
Target: 5'- gGgGUGCcUCGGGCaggacGGGCCGcUCGGGCa -3' miRNA: 3'- aUgCGCGcAGCUCG-----UCCGGU-AGUCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 27520 | 0.67 | 0.543796 |
Target: 5'- gACGC-CGUCGAGCAcgaccucggcgguGGCgGgCAGACc -3' miRNA: 3'- aUGCGcGCAGCUCGU-------------CCGgUaGUCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 23918 | 0.67 | 0.534371 |
Target: 5'- gUugGUGCuGUCGAGCGuGCCcguguagccgGUCGGGCc -3' miRNA: 3'- -AugCGCG-CAGCUCGUcCGG----------UAGUCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 58252 | 0.67 | 0.510562 |
Target: 5'- cGCGCuGCcgggcgaucaucauGUCGGGCGGGUCAUCgagguGGGCc -3' miRNA: 3'- aUGCG-CG--------------CAGCUCGUCCGGUAG-----UCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 11548 | 0.68 | 0.503402 |
Target: 5'- gUugGUGCGaCGcugcGGCAGGCCGguggcacCGGACa -3' miRNA: 3'- -AugCGCGCaGC----UCGUCCGGUa------GUCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 36811 | 0.68 | 0.483196 |
Target: 5'- cUGCGCGCcUCGGucuGCGacuuGGCC-UCAGACa -3' miRNA: 3'- -AUGCGCGcAGCU---CGU----CCGGuAGUCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 2767 | 0.68 | 0.483196 |
Target: 5'- cACGCG-GUCGuGCA-GCCAUC-GACg -3' miRNA: 3'- aUGCGCgCAGCuCGUcCGGUAGuCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 6155 | 0.68 | 0.483196 |
Target: 5'- aGCGCGCGguugaCGAGCAG-CCGcUCGGcGCg -3' miRNA: 3'- aUGCGCGCa----GCUCGUCcGGU-AGUC-UG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 22045 | 0.68 | 0.473239 |
Target: 5'- gAC-CGCGUUGAGCuuguuGGCCAcgccgccgaugaUCGGGCc -3' miRNA: 3'- aUGcGCGCAGCUCGu----CCGGU------------AGUCUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 59104 | 0.69 | 0.434497 |
Target: 5'- cGCGCGCGaCGAGCuuucGGCCcUCGcGCu -3' miRNA: 3'- aUGCGCGCaGCUCGu---CCGGuAGUcUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 27627 | 0.69 | 0.425101 |
Target: 5'- aGCGUGCgGUCGAGCuuGGCCGguaccCAuGGCg -3' miRNA: 3'- aUGCGCG-CAGCUCGu-CCGGUa----GU-CUG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 15381 | 0.69 | 0.397657 |
Target: 5'- --aGCagGCGUCGAGCGGuGCCGUUGGcCg -3' miRNA: 3'- augCG--CGCAGCUCGUC-CGGUAGUCuG- -5' |
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23189 | 5' | -57.9 | NC_005259.1 | + | 58750 | 0.69 | 0.397657 |
Target: 5'- gGCG-GUGUgcuugaUGAGCGGGCCGgucUCAGGCa -3' miRNA: 3'- aUGCgCGCA------GCUCGUCCGGU---AGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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