Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23190 | 3' | -54.3 | NC_005259.1 | + | 27523 | 0.67 | 0.772676 |
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Target: 5'- cGUGAcGCCGucgagcacgaccucgGCGGUGgcgGgCAGACCGu -3' miRNA: 3'- cCACU-CGGU---------------UGCCACa--UgGUCUGGCu -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 9719 | 0.67 | 0.778599 |
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Target: 5'- --aGA-CCAGCGGUGUG-CAGACCu- -3' miRNA: 3'- ccaCUcGGUUGCCACAUgGUCUGGcu -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 32176 | 0.67 | 0.807372 |
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Target: 5'- cGGUGAGaCCcACGGuUGUGCaCGuGCUGAc -3' miRNA: 3'- -CCACUC-GGuUGCC-ACAUG-GUcUGGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 37648 | 0.67 | 0.807372 |
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Target: 5'- cGGUGuGaCCGACGaUGuUACCgAGACCGu -3' miRNA: 3'- -CCACuC-GGUUGCcAC-AUGG-UCUGGCu -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 18836 | 0.66 | 0.815705 |
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Target: 5'- cGUGAGCgAGCGGUGgaaUGCCcgcAGAugcucgcCCGAc -3' miRNA: 3'- cCACUCGgUUGCCAC---AUGG---UCU-------GGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 35121 | 0.66 | 0.816621 |
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Target: 5'- cGGUGAGCaCAcGCGG-GaACCacccgcgcgacGGGCCGAg -3' miRNA: 3'- -CCACUCG-GU-UGCCaCaUGG-----------UCUGGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 62446 | 0.66 | 0.825682 |
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Target: 5'- cGGUGaAGCUGugcGCGGcGUGUCAGGCCGc -3' miRNA: 3'- -CCAC-UCGGU---UGCCaCAUGGUCUGGCu -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 19441 | 0.66 | 0.825682 |
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Target: 5'- --cGGGCUGuucguCGGUcGUgGCCAGACCGGc -3' miRNA: 3'- ccaCUCGGUu----GCCA-CA-UGGUCUGGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 7497 | 0.66 | 0.851648 |
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Target: 5'- -uUGAGgCGcuCGGUGUGCCGG-UCGAc -3' miRNA: 3'- ccACUCgGUu-GCCACAUGGUCuGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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