Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23190 | 3' | -54.3 | NC_005259.1 | + | 7405 | 0.72 | 0.514364 |
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Target: 5'- cGGUGGGCCggUGGgGUcggcggggaacAgCAGACCGGc -3' miRNA: 3'- -CCACUCGGuuGCCaCA-----------UgGUCUGGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 53861 | 0.72 | 0.514364 |
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Target: 5'- aGGUGuacggGGCCAccuCGGUG-GCCgAGGCCGAg -3' miRNA: 3'- -CCAC-----UCGGUu--GCCACaUGG-UCUGGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 19100 | 0.72 | 0.504081 |
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Target: 5'- aGGUcGuGCCcgcguacCGGgGUGCCAGACCGAu -3' miRNA: 3'- -CCA-CuCGGuu-----GCCaCAUGGUCUGGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 67655 | 0.72 | 0.493888 |
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Target: 5'- --cGAGCgCAACGGUGccUACUGGGCCGc -3' miRNA: 3'- ccaCUCG-GUUGCCAC--AUGGUCUGGCu -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 10432 | 0.72 | 0.483791 |
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Target: 5'- cGGuUGGGCCGgaucGCGGcgcgGcUGCCGGGCCGGa -3' miRNA: 3'- -CC-ACUCGGU----UGCCa---C-AUGGUCUGGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 66849 | 0.73 | 0.425457 |
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Target: 5'- cGGUGcuGGCCAGCGGcGUcgagccGCCGGuuGCCGAg -3' miRNA: 3'- -CCAC--UCGGUUGCCaCA------UGGUC--UGGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 52774 | 0.74 | 0.406961 |
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Target: 5'- aGGUGGGCCGAacgcacccCGGUGcUGCgCAccGACCGAg -3' miRNA: 3'- -CCACUCGGUU--------GCCAC-AUG-GU--CUGGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 58758 | 0.76 | 0.307189 |
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Target: 5'- -cUGAGCaCGGCGGUGUGCUugaugagcGGGCCGGu -3' miRNA: 3'- ccACUCG-GUUGCCACAUGG--------UCUGGCU- -5' |
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| 23190 | 3' | -54.3 | NC_005259.1 | + | 55786 | 0.78 | 0.227233 |
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Target: 5'- --gGGGCCAGCGGUGgGCCucGACCGGc -3' miRNA: 3'- ccaCUCGGUUGCCACaUGGu-CUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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