Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23195 | 3' | -56.8 | NC_005259.1 | + | 38746 | 0.66 | 0.690627 |
Target: 5'- gGUGGCC--CGCCGCAggccgGUG-AGCUg -3' miRNA: 3'- gCACCGGucGUGGCGUaa---CGCaUCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 9818 | 0.66 | 0.679894 |
Target: 5'- ---cGCCcGCACCGCcgggUGCGgcGCUg -3' miRNA: 3'- gcacCGGuCGUGGCGua--ACGCauCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 15393 | 0.66 | 0.679894 |
Target: 5'- cCGUGGCCGcGCACUcccaaggguGCGcUGCGU-GCc -3' miRNA: 3'- -GCACCGGU-CGUGG---------CGUaACGCAuCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 28292 | 0.66 | 0.679894 |
Target: 5'- uGUaGGCCacGGUGCCGCccUGCGcAGCg -3' miRNA: 3'- gCA-CCGG--UCGUGGCGuaACGCaUCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 56171 | 0.66 | 0.678818 |
Target: 5'- uCGuUGGCCacGGUGCCgacauacGCGUUGCGcgAGCUg -3' miRNA: 3'- -GC-ACCGG--UCGUGG-------CGUAACGCa-UCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 1439 | 0.66 | 0.669116 |
Target: 5'- uCG-GGCUGGCACUGCuc-GCGU-GCUu -3' miRNA: 3'- -GCaCCGGUCGUGGCGuaaCGCAuCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 26353 | 0.66 | 0.658305 |
Target: 5'- uCG-GGCCgAGCugCGCGUagaUGUcgGUGGCg -3' miRNA: 3'- -GCaCCGG-UCGugGCGUA---ACG--CAUCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 67984 | 0.66 | 0.658305 |
Target: 5'- gCGUGGCCAcCGCCGCGa-GCGUcgacuuGCc -3' miRNA: 3'- -GCACCGGUcGUGGCGUaaCGCAu-----CGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 48052 | 0.66 | 0.658305 |
Target: 5'- gGUGGCCacggcggguAGCGCCGCc--GCGcccugAGCg -3' miRNA: 3'- gCACCGG---------UCGUGGCGuaaCGCa----UCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 39820 | 0.66 | 0.658305 |
Target: 5'- -uUGGCCAGCGCauugagCGCGUUG-GUcGGCg -3' miRNA: 3'- gcACCGGUCGUG------GCGUAACgCA-UCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 26814 | 0.66 | 0.636622 |
Target: 5'- -uUGGCCGGUACCGgCGguggUGCcggGGCg -3' miRNA: 3'- gcACCGGUCGUGGC-GUa---ACGca-UCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 42514 | 0.66 | 0.636622 |
Target: 5'- uGUGGCCAGCAgCCGUccgGCcccgaugGGCa -3' miRNA: 3'- gCACCGGUCGU-GGCGuaaCGca-----UCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 2019 | 0.67 | 0.625769 |
Target: 5'- gGUGGCgcacCGGCACCuCAgUGCGgugGGCg -3' miRNA: 3'- gCACCG----GUCGUGGcGUaACGCa--UCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 54303 | 0.67 | 0.614922 |
Target: 5'- --aGGCCAGCACCGU---GCacGGCUa -3' miRNA: 3'- gcaCCGGUCGUGGCGuaaCGcaUCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 8770 | 0.68 | 0.561078 |
Target: 5'- -uUGGCCAGCGCgGCggUGaugAGCUc -3' miRNA: 3'- gcACCGGUCGUGgCGuaACgcaUCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 23596 | 0.68 | 0.550445 |
Target: 5'- gCGUGGCaCGGCAguuccCCGCGUgGCGgcAGCc -3' miRNA: 3'- -GCACCG-GUCGU-----GGCGUAaCGCa-UCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 41663 | 0.69 | 0.498349 |
Target: 5'- gGUGaGCgGgaucuGCACCGCGU-GCGUGGCc -3' miRNA: 3'- gCAC-CGgU-----CGUGGCGUAaCGCAUCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 22208 | 0.69 | 0.488187 |
Target: 5'- aCGuUGGCCAccgcGCACCGCc--GCGaGGCUg -3' miRNA: 3'- -GC-ACCGGU----CGUGGCGuaaCGCaUCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 36236 | 0.7 | 0.448571 |
Target: 5'- -uUGGCCAGCGCgaGCGccUGCGUcGGCa -3' miRNA: 3'- gcACCGGUCGUGg-CGUa-ACGCA-UCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 35663 | 0.7 | 0.420057 |
Target: 5'- gGUGGCCAGCGCgGUGguggUGCacAGCg -3' miRNA: 3'- gCACCGGUCGUGgCGUa---ACGcaUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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