Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23195 | 3' | -56.8 | NC_005259.1 | + | 63866 | 0.71 | 0.383799 |
Target: 5'- gGUGGCCGGCugGCCuuGCGUggGCGUGGa- -3' miRNA: 3'- gCACCGGUCG--UGG--CGUAa-CGCAUCga -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 58450 | 0.71 | 0.358003 |
Target: 5'- gGUGGCCuuGGUgAUCGCGgccaccagUGCGUGGCUg -3' miRNA: 3'- gCACCGG--UCG-UGGCGUa-------ACGCAUCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 45511 | 0.72 | 0.341493 |
Target: 5'- uGUGGCCAccGCGCCGgUGUUGgccgcCGUGGCUg -3' miRNA: 3'- gCACCGGU--CGUGGC-GUAAC-----GCAUCGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 45782 | 0.72 | 0.32554 |
Target: 5'- gGU-GCCAGCACCGCGUUGaCGguguccuGCUu -3' miRNA: 3'- gCAcCGGUCGUGGCGUAAC-GCau-----CGA- -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 45336 | 0.75 | 0.20564 |
Target: 5'- gCGUGGCCuGCGCCGCcugggccgccGCGUAGUc -3' miRNA: 3'- -GCACCGGuCGUGGCGuaa-------CGCAUCGa -5' |
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23195 | 3' | -56.8 | NC_005259.1 | + | 62627 | 1.07 | 0.001078 |
Target: 5'- cCGUGGCCAGCACCGCAUUGCGUAGCUu -3' miRNA: 3'- -GCACCGGUCGUGGCGUAACGCAUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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