Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23195 | 3' | -56.8 | NC_005259.1 | + | 22208 | 0.69 | 0.488187 |
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Target: 5'- aCGuUGGCCAccgcGCACCGCc--GCGaGGCUg -3' miRNA: 3'- -GC-ACCGGU----CGUGGCGuaaCGCaUCGA- -5' |
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| 23195 | 3' | -56.8 | NC_005259.1 | + | 15393 | 0.66 | 0.679894 |
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Target: 5'- cCGUGGCCGcGCACUcccaaggguGCGcUGCGU-GCc -3' miRNA: 3'- -GCACCGGU-CGUGG---------CGUaACGCAuCGa -5' |
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| 23195 | 3' | -56.8 | NC_005259.1 | + | 9818 | 0.66 | 0.679894 |
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Target: 5'- ---cGCCcGCACCGCcgggUGCGgcGCUg -3' miRNA: 3'- gcacCGGuCGUGGCGua--ACGCauCGA- -5' |
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| 23195 | 3' | -56.8 | NC_005259.1 | + | 8770 | 0.68 | 0.561078 |
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Target: 5'- -uUGGCCAGCGCgGCggUGaugAGCUc -3' miRNA: 3'- gcACCGGUCGUGgCGuaACgcaUCGA- -5' |
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| 23195 | 3' | -56.8 | NC_005259.1 | + | 2019 | 0.67 | 0.625769 |
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Target: 5'- gGUGGCgcacCGGCACCuCAgUGCGgugGGCg -3' miRNA: 3'- gCACCG----GUCGUGGcGUaACGCa--UCGa -5' |
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| 23195 | 3' | -56.8 | NC_005259.1 | + | 1439 | 0.66 | 0.669116 |
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Target: 5'- uCG-GGCUGGCACUGCuc-GCGU-GCUu -3' miRNA: 3'- -GCaCCGGUCGUGGCGuaaCGCAuCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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