Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23198 | 3' | -49.3 | NC_005259.1 | + | 56553 | 0.66 | 0.983645 |
Target: 5'- cUCGUCGGUcucgGGUucg--GUGAucGUCGCGg -3' miRNA: 3'- -GGUAGCCG----CCGuuuuaCACU--UAGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 48950 | 0.66 | 0.983645 |
Target: 5'- aCCGUCGGUuacaucGGCAAcgcccgcaUGggUCGUGa -3' miRNA: 3'- -GGUAGCCG------CCGUUuuac----ACuuAGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 64813 | 0.66 | 0.981496 |
Target: 5'- gCC-UCGGCGGCAAugagcGUGUGG--UGaCGg -3' miRNA: 3'- -GGuAGCCGCCGUUu----UACACUuaGC-GC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 39093 | 0.66 | 0.981496 |
Target: 5'- uCCGUUGaGCGGguuGAGGUGcUGcAAUCGCGu -3' miRNA: 3'- -GGUAGC-CGCCg--UUUUAC-AC-UUAGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 60963 | 0.66 | 0.98127 |
Target: 5'- gCGUcCGGCGGCAGAucgaaccAUuccucuucgGUGAGcUCGCGc -3' miRNA: 3'- gGUA-GCCGCCGUUU-------UA---------CACUU-AGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 27405 | 0.66 | 0.979141 |
Target: 5'- gCAgCGGCGGCAGcucgGUGcc-CGCGa -3' miRNA: 3'- gGUaGCCGCCGUUuua-CACuuaGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 50276 | 0.66 | 0.976572 |
Target: 5'- gUCGUCGGUGGCGGGAac---GUCGUGc -3' miRNA: 3'- -GGUAGCCGCCGUUUUacacuUAGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 49063 | 0.66 | 0.974067 |
Target: 5'- -gAUCGGCGGUuggcgcaagGGAUCGCu -3' miRNA: 3'- ggUAGCCGCCGuuuuaca--CUUAGCGc -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 57017 | 0.66 | 0.973777 |
Target: 5'- cUCAgcgCGGCucGGCGcgGUG-GggUCGCa -3' miRNA: 3'- -GGUa--GCCG--CCGUuuUACaCuuAGCGc -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 42608 | 0.66 | 0.973777 |
Target: 5'- aCAUCGGCGGuCAuGAgcccgauGUCGCGc -3' miRNA: 3'- gGUAGCCGCC-GUuUUacacu--UAGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 26374 | 0.67 | 0.967477 |
Target: 5'- -uGUCGGUGGCGAGccUGUugcUCGCGa -3' miRNA: 3'- ggUAGCCGCCGUUUu-ACAcuuAGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 20650 | 0.67 | 0.957777 |
Target: 5'- aCguUCGGCGGCAccgGggccgaggacgccauGAUGUG-GUCGCa -3' miRNA: 3'- -GguAGCCGCCGU---U---------------UUACACuUAGCGc -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 49629 | 0.68 | 0.947217 |
Target: 5'- gUCAUCGGCGGCAGccgcgucggcGAUGgcgGccucGAUaCGCGc -3' miRNA: 3'- -GGUAGCCGCCGUU----------UUACa--C----UUA-GCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 41496 | 0.68 | 0.946742 |
Target: 5'- aCC-UCGGCgguggcgGGCAGAccGUGGAUCuGCa -3' miRNA: 3'- -GGuAGCCG-------CCGUUUuaCACUUAG-CGc -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 41104 | 0.68 | 0.937188 |
Target: 5'- gCgGUCGGCGGUAugacGAUGUcGGcggugcccgcGUCGCGc -3' miRNA: 3'- -GgUAGCCGCCGUu---UUACA-CU----------UAGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 47788 | 0.69 | 0.907116 |
Target: 5'- -aAUCGcGCaGCGguccGAcgGUGAGUCGCGa -3' miRNA: 3'- ggUAGC-CGcCGU----UUuaCACUUAGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 44865 | 0.69 | 0.900248 |
Target: 5'- cUCAgacCGGCGGCGAGAUccGAcgCGCc -3' miRNA: 3'- -GGUa--GCCGCCGUUUUAcaCUuaGCGc -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 39130 | 0.7 | 0.893101 |
Target: 5'- aCAUCGGUGaGCugcccGAUGUG-AUCGCc -3' miRNA: 3'- gGUAGCCGC-CGuu---UUACACuUAGCGc -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 62738 | 0.71 | 0.853368 |
Target: 5'- aCCGgacCGGCGGCAGuacGcgGUGGuacUCGUGg -3' miRNA: 3'- -GGUa--GCCGCCGUU---UuaCACUu--AGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 37088 | 0.71 | 0.853368 |
Target: 5'- aCCggCGGUGGUguAGAcgcGUGUGAuAUCGCGc -3' miRNA: 3'- -GGuaGCCGCCG--UUU---UACACU-UAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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