Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23198 | 3' | -49.3 | NC_005259.1 | + | 58446 | 0.71 | 0.844669 |
Target: 5'- -gAUCGGUGGCcuug-GUG-AUCGCGg -3' miRNA: 3'- ggUAGCCGCCGuuuuaCACuUAGCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 54496 | 0.72 | 0.807654 |
Target: 5'- gUCAUCGGCgGGCAuggcggcGAUGUgGggUgGCGg -3' miRNA: 3'- -GGUAGCCG-CCGUu------UUACA-CuuAgCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 6537 | 0.72 | 0.787959 |
Target: 5'- aCCGgccUCGG-GGCGAGcgGUGAAgcugugCGCGg -3' miRNA: 3'- -GGU---AGCCgCCGUUUuaCACUUa-----GCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 33320 | 0.72 | 0.777853 |
Target: 5'- gCCGUCGGCGGUcacgcgGAAGUGaucgGggUCGa- -3' miRNA: 3'- -GGUAGCCGCCG------UUUUACa---CuuAGCgc -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 4478 | 0.78 | 0.49394 |
Target: 5'- gCCAUCGGCGGUGGAccaugGUG-AUCGCc -3' miRNA: 3'- -GGUAGCCGCCGUUUua---CACuUAGCGc -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 28233 | 0.8 | 0.36763 |
Target: 5'- aCCGUCGGCGGCGGGGUGgucGAUCaCGa -3' miRNA: 3'- -GGUAGCCGCCGUUUUACac-UUAGcGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 60603 | 0.81 | 0.332654 |
Target: 5'- gCCGUCaucgagcagcacaGGCGGCGAGGUGUGGAacugCGCGu -3' miRNA: 3'- -GGUAG-------------CCGCCGUUUUACACUUa---GCGC- -5' |
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23198 | 3' | -49.3 | NC_005259.1 | + | 61673 | 1.13 | 0.003281 |
Target: 5'- cCCAUCGGCGGCAAAAUGUGAAUCGCGg -3' miRNA: 3'- -GGUAGCCGCCGUUUUACACUUAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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