Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23198 | 3' | -49.3 | NC_005259.1 | + | 37088 | 0.71 | 0.853368 |
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Target: 5'- aCCggCGGUGGUguAGAcgcGUGUGAuAUCGCGc -3' miRNA: 3'- -GGuaGCCGCCG--UUU---UACACU-UAGCGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 33320 | 0.72 | 0.777853 |
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Target: 5'- gCCGUCGGCGGUcacgcgGAAGUGaucgGggUCGa- -3' miRNA: 3'- -GGUAGCCGCCG------UUUUACa---CuuAGCgc -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 28233 | 0.8 | 0.36763 |
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Target: 5'- aCCGUCGGCGGCGGGGUGgucGAUCaCGa -3' miRNA: 3'- -GGUAGCCGCCGUUUUACac-UUAGcGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 27405 | 0.66 | 0.979141 |
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Target: 5'- gCAgCGGCGGCAGcucgGUGcc-CGCGa -3' miRNA: 3'- gGUaGCCGCCGUUuua-CACuuaGCGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 26374 | 0.67 | 0.967477 |
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Target: 5'- -uGUCGGUGGCGAGccUGUugcUCGCGa -3' miRNA: 3'- ggUAGCCGCCGUUUu-ACAcuuAGCGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 20650 | 0.67 | 0.957777 |
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Target: 5'- aCguUCGGCGGCAccgGggccgaggacgccauGAUGUG-GUCGCa -3' miRNA: 3'- -GguAGCCGCCGU---U---------------UUACACuUAGCGc -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 6537 | 0.72 | 0.787959 |
|
Target: 5'- aCCGgccUCGG-GGCGAGcgGUGAAgcugugCGCGg -3' miRNA: 3'- -GGU---AGCCgCCGUUUuaCACUUa-----GCGC- -5' |
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| 23198 | 3' | -49.3 | NC_005259.1 | + | 4478 | 0.78 | 0.49394 |
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Target: 5'- gCCAUCGGCGGUGGAccaugGUG-AUCGCc -3' miRNA: 3'- -GGUAGCCGCCGUUUua---CACuUAGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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