Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23199 | 3' | -53.2 | NC_005259.1 | + | 55241 | 0.66 | 0.913197 |
Target: 5'- uGCCca-CGACCUaUGUCGGGG---UCCa -3' miRNA: 3'- -CGGccaGCUGGA-GCAGCCCUuaaAGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 26281 | 0.66 | 0.913197 |
Target: 5'- cGCCGGUgcCGcCCUCGacggguaCGGGGAUcgacCCa -3' miRNA: 3'- -CGGCCA--GCuGGAGCa------GCCCUUAaa--GG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 13698 | 0.66 | 0.909426 |
Target: 5'- gGCCGGUCGACCcCGccucgacaccgacaaUCGaGGug---CCg -3' miRNA: 3'- -CGGCCAGCUGGaGC---------------AGC-CCuuaaaGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 56154 | 0.66 | 0.890599 |
Target: 5'- uCCGGUCGAgcacguCCUCGUUGGccacggugCCg -3' miRNA: 3'- cGGCCAGCU------GGAGCAGCCcuuaaa--GG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 30803 | 0.66 | 0.886305 |
Target: 5'- cGCCGGaacACCUgaGUCGGGuag-UCCg -3' miRNA: 3'- -CGGCCagcUGGAg-CAGCCCuuaaAGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 18706 | 0.67 | 0.871354 |
Target: 5'- cGgCGGUCuGACCUCGaucaUCGGGccgc-CCg -3' miRNA: 3'- -CgGCCAG-CUGGAGC----AGCCCuuaaaGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 22531 | 0.67 | 0.868248 |
Target: 5'- cGCCGGUgauuuccagcagucCGACCUCGcCGaggucauGGAAg--CCg -3' miRNA: 3'- -CGGCCA--------------GCUGGAGCaGC-------CCUUaaaGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 43184 | 0.67 | 0.847166 |
Target: 5'- aGUCGGUacCGACCUCGgCGGcGAug-UCg -3' miRNA: 3'- -CGGCCA--GCUGGAGCaGCC-CUuaaAGg -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 9556 | 0.67 | 0.847166 |
Target: 5'- cCCGGccUCGACCgCGUCGGcGAGgugCUc -3' miRNA: 3'- cGGCC--AGCUGGaGCAGCC-CUUaaaGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 283 | 0.67 | 0.84379 |
Target: 5'- cGCCGccuacgCGGCCUCGcgcugaucucgacCGGGggUgcgUCCg -3' miRNA: 3'- -CGGCca----GCUGGAGCa------------GCCCuuAa--AGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 22387 | 0.68 | 0.838662 |
Target: 5'- cCCGGUCGGCCUCGgcgacgugaUCGaGAAagUCg -3' miRNA: 3'- cGGCCAGCUGGAGC---------AGCcCUUaaAGg -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 41644 | 0.68 | 0.838662 |
Target: 5'- uGCCGGUCugcgGGCgCUCGgugagCGGGA---UCUg -3' miRNA: 3'- -CGGCCAG----CUG-GAGCa----GCCCUuaaAGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 8358 | 0.68 | 0.829951 |
Target: 5'- uGCCGGUaucgcucggcggCGGCCUCGgcaUCGGcGAGag-CCu -3' miRNA: 3'- -CGGCCA------------GCUGGAGC---AGCC-CUUaaaGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 67430 | 0.68 | 0.81194 |
Target: 5'- uGCCGGgcucacgcUUGAgCgCGUCGGGGAUgcCCu -3' miRNA: 3'- -CGGCC--------AGCUgGaGCAGCCCUUAaaGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 41315 | 0.68 | 0.793208 |
Target: 5'- cGCgaGGUCGACCUCGaaaaGGGcggugCCg -3' miRNA: 3'- -CGg-CCAGCUGGAGCag--CCCuuaaaGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 63869 | 0.69 | 0.783596 |
Target: 5'- gGCCGGcUGGCCUUGcgUGGGcGUggaUUCCa -3' miRNA: 3'- -CGGCCaGCUGGAGCa-GCCCuUA---AAGG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 41267 | 0.69 | 0.773834 |
Target: 5'- cCCGGUCGAgcacCCUCGUCGGcgcacUCa -3' miRNA: 3'- cGGCCAGCU----GGAGCAGCCcuuaaAGg -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 64215 | 0.69 | 0.773834 |
Target: 5'- cGCCGGUCGGCagccCGguaUCGGGGucgauagCCg -3' miRNA: 3'- -CGGCCAGCUGga--GC---AGCCCUuaaa---GG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 60282 | 0.69 | 0.763931 |
Target: 5'- uGCCGGgCGGCCaUCGUCGucGGUgcggCCa -3' miRNA: 3'- -CGGCCaGCUGG-AGCAGCccUUAaa--GG- -5' |
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23199 | 3' | -53.2 | NC_005259.1 | + | 58788 | 0.69 | 0.763931 |
Target: 5'- cGCCGGUCGaggcggugauuGCCUCGgCGGGcucgcgCUg -3' miRNA: 3'- -CGGCCAGC-----------UGGAGCaGCCCuuaaa-GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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