Results 1 - 20 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23199 | 5' | -57.4 | NC_005259.1 | + | 49351 | 0.66 | 0.694373 |
Target: 5'- gGCGAGAgGUGCCG-CGCGaugaagagGCCGGUg -3' miRNA: 3'- -CGCUCU-UACGGCuGUGCcg------UGGCCGu -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 47009 | 0.66 | 0.694373 |
Target: 5'- cGCGAGGAaGCCGAcCACGaccgccuccaGCagGCCGGgGa -3' miRNA: 3'- -CGCUCUUaCGGCU-GUGC----------CG--UGGCCgU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 44463 | 0.66 | 0.694373 |
Target: 5'- cCGGGGAUGCCGcCGCccuGGC-CgGGCc -3' miRNA: 3'- cGCUCUUACGGCuGUG---CCGuGgCCGu -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 17757 | 0.66 | 0.694373 |
Target: 5'- cGUGAGGccGCCG-CGCGccgggccgagcGCACCGaGCGu -3' miRNA: 3'- -CGCUCUuaCGGCuGUGC-----------CGUGGC-CGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 6696 | 0.66 | 0.683846 |
Target: 5'- -aGAGug-GCgCGACACGaucaACCGGCAg -3' miRNA: 3'- cgCUCuuaCG-GCUGUGCcg--UGGCCGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 62720 | 0.66 | 0.683846 |
Target: 5'- gGCGGGGGaucucGCuCGAC-CGG-ACCGGCGg -3' miRNA: 3'- -CGCUCUUa----CG-GCUGuGCCgUGGCCGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 35703 | 0.66 | 0.672212 |
Target: 5'- gGCGAGGGgucGCUGACGaGGUAuccgcccgacgacCCGGCGc -3' miRNA: 3'- -CGCUCUUa--CGGCUGUgCCGU-------------GGCCGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 13813 | 0.66 | 0.670092 |
Target: 5'- gGCGAGcaccacgugugcguGAUGaCCGACG-GGCAcauugcCCGGCGa -3' miRNA: 3'- -CGCUC--------------UUAC-GGCUGUgCCGU------GGCCGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 24083 | 0.66 | 0.66691 |
Target: 5'- cGCGAGAucaCCGGCAgGGCcaacgaaccgaugggACCGGgCGg -3' miRNA: 3'- -CGCUCUuacGGCUGUgCCG---------------UGGCC-GU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 54689 | 0.66 | 0.662662 |
Target: 5'- aGCGAGAccGUGUCGAgGCGGguggccucaGCCuGGUAc -3' miRNA: 3'- -CGCUCU--UACGGCUgUGCCg--------UGG-CCGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 28967 | 0.66 | 0.662662 |
Target: 5'- cCGAGGucgGUGCCGAggcCGCGaGCGCCcGCu -3' miRNA: 3'- cGCUCU---UACGGCU---GUGC-CGUGGcCGu -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 8076 | 0.66 | 0.662662 |
Target: 5'- uCGAGAucGUGCCG-CGCuGGCgugACCGuGCGa -3' miRNA: 3'- cGCUCU--UACGGCuGUG-CCG---UGGC-CGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 4263 | 0.66 | 0.641371 |
Target: 5'- uGCGAGGAcaacgggcgGCUGcgcugccacgaGCACGG-ACCGGCGg -3' miRNA: 3'- -CGCUCUUa--------CGGC-----------UGUGCCgUGGCCGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 38441 | 0.66 | 0.641371 |
Target: 5'- uCGAGAccgaaaAUGCCGuGCGagGGCACCgagGGCAg -3' miRNA: 3'- cGCUCU------UACGGC-UGUg-CCGUGG---CCGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 50678 | 0.66 | 0.641371 |
Target: 5'- cGCGucGGUGCCGACGaGGCGCU-GCu -3' miRNA: 3'- -CGCucUUACGGCUGUgCCGUGGcCGu -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 33105 | 0.67 | 0.598765 |
Target: 5'- cGCGAGcGUGCCuguagggccACGCGGCagccGCCGcGCGu -3' miRNA: 3'- -CGCUCuUACGGc--------UGUGCCG----UGGC-CGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 30745 | 0.67 | 0.576534 |
Target: 5'- cUGAGAguucaugaacugcAUGCCGACGCcgguGGCACCGcuGCc -3' miRNA: 3'- cGCUCU-------------UACGGCUGUG----CCGUGGC--CGu -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 57297 | 0.68 | 0.56706 |
Target: 5'- uCGu-AGUGCaCGACAuCGaGCACCGGCGg -3' miRNA: 3'- cGCucUUACG-GCUGU-GC-CGUGGCCGU- -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 29933 | 0.68 | 0.556581 |
Target: 5'- cGCGuGGccGCC-ACGCGGC-CCGGUc -3' miRNA: 3'- -CGCuCUuaCGGcUGUGCCGuGGCCGu -5' |
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23199 | 5' | -57.4 | NC_005259.1 | + | 45119 | 0.68 | 0.546158 |
Target: 5'- cCGAGAgugGUGCCGACGaGGU--CGGCAa -3' miRNA: 3'- cGCUCU---UACGGCUGUgCCGugGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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