Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23203 | 3' | -56.4 | NC_005259.1 | + | 68091 | 0.7 | 0.462272 |
Target: 5'- aGCUCGAuGGcCGGgGuaacgacguaguUCGGGUCGAGaGCa -3' miRNA: 3'- -CGAGUU-CC-GCCgCu-----------AGUCCAGCUC-CG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 26691 | 0.66 | 0.749769 |
Target: 5'- -aUCGAGGCGGgGAacUCGgGGUUGAuuuccucgaccaucgGGCc -3' miRNA: 3'- cgAGUUCCGCCgCU--AGU-CCAGCU---------------CCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 778 | 0.68 | 0.618192 |
Target: 5'- cGC-CGAGGCGGCugcccucGGUUGGGUggccaaGGGGCc -3' miRNA: 3'- -CGaGUUCCGCCG-------CUAGUCCAg-----CUCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 23285 | 0.68 | 0.597893 |
Target: 5'- --cCGGGcGCGGUGGUUGGGuaUCGGGGUc -3' miRNA: 3'- cgaGUUC-CGCCGCUAGUCC--AGCUCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 16676 | 0.68 | 0.597893 |
Target: 5'- cCUCGAuguGGuCGGCGAUCcuGGGcacCGAGGUg -3' miRNA: 3'- cGAGUU---CC-GCCGCUAG--UCCa--GCUCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 17171 | 0.69 | 0.555546 |
Target: 5'- aGCUgacgaCAAGGCGGCG--CAGGaagccgcgcgUGAGGCg -3' miRNA: 3'- -CGA-----GUUCCGCCGCuaGUCCa---------GCUCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 25413 | 0.69 | 0.545085 |
Target: 5'- cCUCAaccGGGaCGGCGA---GGUCGAGGa -3' miRNA: 3'- cGAGU---UCC-GCCGCUaguCCAGCUCCg -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 51139 | 0.69 | 0.534689 |
Target: 5'- -gUCGGGGCGGCcauGAgcgcguUCGGGUUGAccguGGCg -3' miRNA: 3'- cgAGUUCCGCCG---CU------AGUCCAGCU----CCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 40037 | 0.7 | 0.518205 |
Target: 5'- uGCuUUGAGGUcgccGGUGAUCGGGUCGgucgcgaugacccauGGGCc -3' miRNA: 3'- -CG-AGUUCCG----CCGCUAGUCCAGC---------------UCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 53009 | 0.7 | 0.493872 |
Target: 5'- cCUCGacuGGGCGcuGCGGUgGcGUCGAGGCa -3' miRNA: 3'- cGAGU---UCCGC--CGCUAgUcCAGCUCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 61027 | 0.7 | 0.493872 |
Target: 5'- aCUCGGuGGCGGCGA---GGUCGAccgacgGGCg -3' miRNA: 3'- cGAGUU-CCGCCGCUaguCCAGCU------CCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 60338 | 1.13 | 0.000594 |
Target: 5'- cGCUCAAGGCGGCGAUCAGGUCGAGGCc -3' miRNA: 3'- -CGAGUUCCGCCGCUAGUCCAGCUCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 54680 | 0.71 | 0.444971 |
Target: 5'- uGCUC--GGCGaGCGAgacCGuGUCGAGGCg -3' miRNA: 3'- -CGAGuuCCGC-CGCUa--GUcCAGCUCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 58772 | 0.71 | 0.426181 |
Target: 5'- cGCUgcGGGCGcuGUGcgCcGGUCGAGGCg -3' miRNA: 3'- -CGAguUCCGC--CGCuaGuCCAGCUCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 54502 | 0.71 | 0.40787 |
Target: 5'- gGCgggCAuGGCGGCGAUgugggguggCGGGUCGAguauccGGCc -3' miRNA: 3'- -CGa--GUuCCGCCGCUA---------GUCCAGCU------CCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 62690 | 0.72 | 0.398902 |
Target: 5'- cCUCGAGuGCggugucccauccGGCGAUCAGG-CGGGGg -3' miRNA: 3'- cGAGUUC-CG------------CCGCUAGUCCaGCUCCg -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 58329 | 0.73 | 0.339773 |
Target: 5'- uCUC-GGGCaGCGAgaUCAGGUCGGuGGCc -3' miRNA: 3'- cGAGuUCCGcCGCU--AGUCCAGCU-CCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 50430 | 0.73 | 0.331863 |
Target: 5'- gGCUC-GGGCaGCGGUgCGGGcUCGGGGUc -3' miRNA: 3'- -CGAGuUCCGcCGCUA-GUCC-AGCUCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 1556 | 0.73 | 0.323318 |
Target: 5'- cGUUCGGGGCGGUGAcgcugugagCgcgccccacaccgAGGUCGAGGUc -3' miRNA: 3'- -CGAGUUCCGCCGCUa--------G-------------UCCAGCUCCG- -5' |
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23203 | 3' | -56.4 | NC_005259.1 | + | 31897 | 0.73 | 0.321783 |
Target: 5'- uCUCAAGGCccGGCGcgagcgccucaAUCucgucacgcacucgGGGUCGAGGCg -3' miRNA: 3'- cGAGUUCCG--CCGC-----------UAG--------------UCCAGCUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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