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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23203 | 5' | -56.6 | NC_005259.1 | + | 15300 | 0.72 | 0.378134 |
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Target: 5'- ----aCCGCCGAccUCGCCGcccacggucucGACGGCGGu -3' miRNA: 3'- uuuagGGCGGUU--AGCGGC-----------CUGUCGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 17763 | 0.72 | 0.378134 |
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Target: 5'- ----gCCGCCGcgCGCCGGGCcgAGCGc -3' miRNA: 3'- uuuagGGCGGUuaGCGGCCUG--UCGCc -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 4981 | 0.72 | 0.344645 |
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Target: 5'- cGAGUUCgGCCGAUgGCCGGGCAcuaCGGc -3' miRNA: 3'- -UUUAGGgCGGUUAgCGGCCUGUc--GCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 47977 | 0.75 | 0.23852 |
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Target: 5'- ---gCCCGCgAAUaUGCCGGGCAGCGc -3' miRNA: 3'- uuuaGGGCGgUUA-GCGGCCUGUCGCc -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 54483 | 0.75 | 0.226638 |
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Target: 5'- -cAUCCCGCCGAgggucaUCGgCGGGCauGGCGGc -3' miRNA: 3'- uuUAGGGCGGUU------AGCgGCCUG--UCGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 33313 | 0.75 | 0.220887 |
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Target: 5'- cGAUCCCGCC-GUCGgCGGuCAcGCGGa -3' miRNA: 3'- uUUAGGGCGGuUAGCgGCCuGU-CGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 45621 | 0.76 | 0.204377 |
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Target: 5'- cGAUgCCCGCCGG--GCCGGGCAGCGc -3' miRNA: 3'- uUUA-GGGCGGUUagCGGCCUGUCGCc -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 47291 | 0.81 | 0.088192 |
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Target: 5'- ----aCCGCC-GUCGCCGGACAGCGa -3' miRNA: 3'- uuuagGGCGGuUAGCGGCCUGUCGCc -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 5706 | 0.85 | 0.050134 |
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Target: 5'- -cGUgCCGCCAAggccagCGCCGGACGGCGGc -3' miRNA: 3'- uuUAgGGCGGUUa-----GCGGCCUGUCGCC- -5' |
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| 23203 | 5' | -56.6 | NC_005259.1 | + | 60372 | 1.07 | 0.001289 |
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Target: 5'- gAAAUCCCGCCAAUCGCCGGACAGCGGc -3' miRNA: 3'- -UUUAGGGCGGUUAGCGGCCUGUCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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