Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23207 | 3' | -57.7 | NC_005259.1 | + | 3299 | 0.68 | 0.525509 |
Target: 5'- cCGGUcccucgGCGAGCgcugcgucgaCGUGAGCGCCggGCa -3' miRNA: 3'- -GCUAcaa---CGCUCG----------GCGCUCGCGGa-CG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 4623 | 0.66 | 0.629643 |
Target: 5'- cCGGUGgcGCGAGuucuggauagugcCCGCGcaccGCGCCUacggGCa -3' miRNA: 3'- -GCUACaaCGCUC-------------GGCGCu---CGCGGA----CG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 8275 | 0.66 | 0.673272 |
Target: 5'- aGGUGUUGUaccGGGUgCGCGGGCaCCcGCa -3' miRNA: 3'- gCUACAACG---CUCG-GCGCUCGcGGaCG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 10783 | 0.73 | 0.274385 |
Target: 5'- uCGAg---GUGGGCCGCGAGCGCaaugucguaguccUUGCg -3' miRNA: 3'- -GCUacaaCGCUCGGCGCUCGCG-------------GACG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 11122 | 0.77 | 0.148226 |
Target: 5'- aCGGUGacggUGCGAGCCGU---CGCCUGCg -3' miRNA: 3'- -GCUACa---ACGCUCGGCGcucGCGGACG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 16511 | 0.66 | 0.673272 |
Target: 5'- cCGAga--GCGAggcGCUGCGAGCGUC-GCu -3' miRNA: 3'- -GCUacaaCGCU---CGGCGCUCGCGGaCG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 20516 | 0.66 | 0.620048 |
Target: 5'- aCGGUGUccCGAGCa-CGGGUGCgCUGCc -3' miRNA: 3'- -GCUACAacGCUCGgcGCUCGCG-GACG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 23321 | 0.7 | 0.434113 |
Target: 5'- uCGGUGUcGcCGAGCCGgauuacaucgggcuCGAGgcUGCCUGCg -3' miRNA: 3'- -GCUACAaC-GCUCGGC--------------GCUC--GCGGACG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 24240 | 0.67 | 0.56706 |
Target: 5'- cCGAggcacGCGAGUUcgGCGAGUGCgUGCa -3' miRNA: 3'- -GCUacaa-CGCUCGG--CGCUCGCGgACG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 25433 | 0.67 | 0.598765 |
Target: 5'- uCGAggaaUGCGAGCCguaugaggucgGCGAGCGUCUu- -3' miRNA: 3'- -GCUaca-ACGCUCGG-----------CGCUCGCGGAcg -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 26481 | 0.67 | 0.577589 |
Target: 5'- aCGuaguUGUcGCGGaacGCCGCGAGCucGCCgcccgGCg -3' miRNA: 3'- -GCu---ACAaCGCU---CGGCGCUCG--CGGa----CG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 28973 | 0.77 | 0.16055 |
Target: 5'- uCGGUGc--CGAgGCCGCGAGCGCCcGCu -3' miRNA: 3'- -GCUACaacGCU-CGGCGCUCGCGGaCG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 29021 | 0.69 | 0.450154 |
Target: 5'- gCGGUGUcugcggucgaacccUGaccaacgacgaGAGCCGCGAGCGCgguaucggcccgauCUGCg -3' miRNA: 3'- -GCUACA--------------ACg----------CUCGGCGCUCGCG--------------GACG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 31732 | 0.72 | 0.318027 |
Target: 5'- aGGUGaccGCGccuGCCGCGAGCGCUgGUg -3' miRNA: 3'- gCUACaa-CGCu--CGGCGCUCGCGGaCG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 35320 | 0.66 | 0.662662 |
Target: 5'- gCGGUGUguacGCGAccCCGCGuAGCGCgUGa -3' miRNA: 3'- -GCUACAa---CGCUc-GGCGC-UCGCGgACg -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 36221 | 0.79 | 0.116263 |
Target: 5'- gCGAUGc--CGAGCaccuuggccagCGCGAGCGCCUGCg -3' miRNA: 3'- -GCUACaacGCUCG-----------GCGCUCGCGGACG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 37564 | 0.68 | 0.525509 |
Target: 5'- cCGAUGUcgacUGCucGCCGCccGCGCCgaucUGCa -3' miRNA: 3'- -GCUACA----ACGcuCGGCGcuCGCGG----ACG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 38932 | 0.71 | 0.352625 |
Target: 5'- uCGGUGUUGCuGugcccggugccgcuGCCGcCGAGaGCCUGCa -3' miRNA: 3'- -GCUACAACG-Cu-------------CGGC-GCUCgCGGACG- -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 39061 | 0.67 | 0.56706 |
Target: 5'- gCGAUGUUGcCGAGCuUGCuGGCGUCg-- -3' miRNA: 3'- -GCUACAAC-GCUCG-GCGcUCGCGGacg -5' |
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23207 | 3' | -57.7 | NC_005259.1 | + | 41424 | 0.71 | 0.382857 |
Target: 5'- aCGAUGUgcaCGGGCCGCcauuuGCGCCccGCg -3' miRNA: 3'- -GCUACAac-GCUCGGCGcu---CGCGGa-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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