Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23210 | 5' | -54.2 | NC_005259.1 | + | 286 | 0.66 | 0.832043 |
Target: 5'- cGCCuacgcggCCUcGCGCUGAUcucgaccgggggugCGUCCggccaaCGCa -3' miRNA: 3'- -CGGua-----GGAaCGCGACUA--------------GCAGGa-----GCG- -5' |
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23210 | 5' | -54.2 | NC_005259.1 | + | 46466 | 0.66 | 0.832043 |
Target: 5'- gGCUcagAUCCUUgaGCGCuguggccacgcccuUGAUCGugUCCUCGUc -3' miRNA: 3'- -CGG---UAGGAA--CGCG--------------ACUAGC--AGGAGCG- -5' |
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23210 | 5' | -54.2 | NC_005259.1 | + | 35907 | 0.66 | 0.825792 |
Target: 5'- aGCCAUUCUgcguUGCGCgGGaCGUCCaCGa -3' miRNA: 3'- -CGGUAGGA----ACGCGaCUaGCAGGaGCg -5' |
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23210 | 5' | -54.2 | NC_005259.1 | + | 59392 | 0.66 | 0.816694 |
Target: 5'- uGCC-UCCgcGCGC-GAgCGUgaCCUCGCg -3' miRNA: 3'- -CGGuAGGaaCGCGaCUaGCA--GGAGCG- -5' |
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23210 | 5' | -54.2 | NC_005259.1 | + | 17351 | 0.66 | 0.851857 |
Target: 5'- cGCCGUCgaggucggcggCUcgGCGCUGGUCGUggUCGa -3' miRNA: 3'- -CGGUAG-----------GAa-CGCGACUAGCAggAGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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