Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23210 | 5' | -54.2 | NC_005259.1 | + | 10976 | 0.66 | 0.834691 |
Target: 5'- uGUgGUCa-UGUGaacGAUUGUCCUCGCa -3' miRNA: 3'- -CGgUAGgaACGCga-CUAGCAGGAGCG- -5' |
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23210 | 5' | -54.2 | NC_005259.1 | + | 48249 | 0.66 | 0.846799 |
Target: 5'- gGUCGUUCUUgGUGCUGuaGUCGUacuuacgguugucgaCCUUGCg -3' miRNA: 3'- -CGGUAGGAA-CGCGAC--UAGCA---------------GGAGCG- -5' |
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23210 | 5' | -54.2 | NC_005259.1 | + | 53007 | 0.66 | 0.846799 |
Target: 5'- uGCCucgaCUggGCGCUGcgguggcgucgaggcAUCGUgaCCUCGCg -3' miRNA: 3'- -CGGua--GGaaCGCGAC---------------UAGCA--GGAGCG- -5' |
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23210 | 5' | -54.2 | NC_005259.1 | + | 56375 | 0.66 | 0.85102 |
Target: 5'- aGCCGUCgCggGCcgcgucgGCUGAUCGU-CUCGa -3' miRNA: 3'- -CGGUAG-GaaCG-------CGACUAGCAgGAGCg -5' |
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23210 | 5' | -54.2 | NC_005259.1 | + | 17351 | 0.66 | 0.851857 |
Target: 5'- cGCCGUCgaggucggcggCUcgGCGCUGGUCGUggUCGa -3' miRNA: 3'- -CGGUAG-----------GAa-CGCGACUAGCAggAGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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