Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23214 | 3' | -58 | NC_005259.1 | + | 14325 | 0.68 | 0.513168 |
Target: 5'- uCGACGccgccgccCGCGUGC-GGGCCGCCg-- -3' miRNA: 3'- -GCUGCuu------GCGCAUGuCCCGGUGGgca -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 13811 | 0.66 | 0.648358 |
Target: 5'- cCGGCGAgcaccACGUGUGCGugaugaccgacGGGCacauuGCCCGg -3' miRNA: 3'- -GCUGCU-----UGCGCAUGU-----------CCCGg----UGGGCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 8666 | 0.69 | 0.483399 |
Target: 5'- gGGCugccACGCGggcaACGGGGCCGCCuCGa -3' miRNA: 3'- gCUGcu--UGCGCa---UGUCCCGGUGG-GCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 8388 | 0.66 | 0.648358 |
Target: 5'- uCGGCGAGagccucgGCGaGCcGGGCCACCuCGc -3' miRNA: 3'- -GCUGCUUg------CGCaUGuCCCGGUGG-GCa -5' |
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23214 | 3' | -58 | NC_005259.1 | + | 1341 | 0.69 | 0.493233 |
Target: 5'- aGAuCGAGCGCaacgGUGCcuacuGGGCCGCUCGc -3' miRNA: 3'- gCU-GCUUGCG----CAUGu----CCCGGUGGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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