Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23215 | 3' | -50.8 | NC_005259.1 | + | 62741 | 0.66 | 0.947632 |
Target: 5'- --gGACCGGCGGCAguacgcgguGGuacuCGUGGGCc -3' miRNA: 3'- gugCUGGUCGUUGU---------UCuu--GCACUCGu -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 44868 | 0.66 | 0.942762 |
Target: 5'- --aGACCGGCGGCGAGAuccgacGCGccccacGAGUAu -3' miRNA: 3'- gugCUGGUCGUUGUUCU------UGCa-----CUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 17505 | 0.66 | 0.937607 |
Target: 5'- gGCGACCuuGCcgaucCGAGcuCGUGGGCAc -3' miRNA: 3'- gUGCUGGu-CGuu---GUUCuuGCACUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 5631 | 0.66 | 0.932163 |
Target: 5'- -cCGACCGGaucgugccCGGCGAGGACGgcGGGCGc -3' miRNA: 3'- guGCUGGUC--------GUUGUUCUUGCa-CUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 3307 | 0.66 | 0.932163 |
Target: 5'- -uCGGCgAGCGcuGCGucGACGUGAGCGc -3' miRNA: 3'- guGCUGgUCGU--UGUucUUGCACUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 50140 | 0.66 | 0.926431 |
Target: 5'- aCGCGAUUgguuGGCGAUccGGcGCGUGAGCGa -3' miRNA: 3'- -GUGCUGG----UCGUUGuuCU-UGCACUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 36392 | 0.66 | 0.926431 |
Target: 5'- -uUGGCCAGC-GCGAGcGACuUGAGCGc -3' miRNA: 3'- guGCUGGUCGuUGUUC-UUGcACUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 63852 | 0.67 | 0.920408 |
Target: 5'- gCGCGuACCGGCGAgGuGGccggcuggccuuGCGUGGGCGu -3' miRNA: 3'- -GUGC-UGGUCGUUgUuCU------------UGCACUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 57468 | 0.67 | 0.920408 |
Target: 5'- gGCaGGCCGGUGGCAccGGAcACGUcGAGCGu -3' miRNA: 3'- gUG-CUGGUCGUUGU--UCU-UGCA-CUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 67834 | 0.67 | 0.914097 |
Target: 5'- -uCGACCGGCAGCccg---GUGAGCGg -3' miRNA: 3'- guGCUGGUCGUUGuucuugCACUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 29041 | 0.67 | 0.914097 |
Target: 5'- -cUGACCAaCGACGAGAGcCGcGAGCGc -3' miRNA: 3'- guGCUGGUcGUUGUUCUU-GCaCUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 64810 | 0.67 | 0.914097 |
Target: 5'- -uCGGCCucGGCGGCAAuGAGCGUGuGGUg -3' miRNA: 3'- guGCUGG--UCGUUGUU-CUUGCAC-UCGu -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 51573 | 0.67 | 0.901317 |
Target: 5'- aACGAuCCGGCAGCcGGugccgacacagccgcACGUGAGCc -3' miRNA: 3'- gUGCU-GGUCGUUGuUCu--------------UGCACUCGu -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 42563 | 0.67 | 0.893451 |
Target: 5'- -uCGGCgAGCAGCcGGucgucaucgauaGGCGUGAGCGa -3' miRNA: 3'- guGCUGgUCGUUGuUC------------UUGCACUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 44051 | 0.67 | 0.89272 |
Target: 5'- gACGACCGGCu-CGAGuGCGcugucguUGAGCu -3' miRNA: 3'- gUGCUGGUCGuuGUUCuUGC-------ACUCGu -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 39836 | 0.68 | 0.88601 |
Target: 5'- gCGCGuuggUCGGCGACAGGAugccgGUGAGCu -3' miRNA: 3'- -GUGCu---GGUCGUUGUUCUug---CACUCGu -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 68311 | 0.68 | 0.878296 |
Target: 5'- gCGCGuCCGGCGACGGGAucuCGaugaugGGGCu -3' miRNA: 3'- -GUGCuGGUCGUUGUUCUu--GCa-----CUCGu -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 60961 | 0.68 | 0.878296 |
Target: 5'- gCGCGuCCGGCGGCAGaucGAACcauuccucuucgGUGAGCu -3' miRNA: 3'- -GUGCuGGUCGUUGUU---CUUG------------CACUCGu -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 30901 | 0.68 | 0.878296 |
Target: 5'- aCGCGagguguGCCGGUu----GAGCGUGAGCAg -3' miRNA: 3'- -GUGC------UGGUCGuuguuCUUGCACUCGU- -5' |
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23215 | 3' | -50.8 | NC_005259.1 | + | 10204 | 0.68 | 0.87751 |
Target: 5'- gACGACCgGGCAgcucgaccaccACGAGAugGcgcucacUGAGCAc -3' miRNA: 3'- gUGCUGG-UCGU-----------UGUUCUugC-------ACUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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