Results 1 - 20 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23216 | 3' | -50.5 | NC_005259.1 | + | 61297 | 0.66 | 0.975714 |
Target: 5'- aCGCGAGCGGcugaucGGUGAAUguccGGAaaagcucugucuUGGCCa -3' miRNA: 3'- gGCGCUUGCCa-----UCGCUUA----UCU------------GCUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 8958 | 0.66 | 0.975714 |
Target: 5'- gCCGUGGACGGgcaccGUGccgGGACGuagguGCCa -3' miRNA: 3'- -GGCGCUUGCCau---CGCuuaUCUGC-----UGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 44296 | 0.66 | 0.972916 |
Target: 5'- gCGCGAGCGGguuGCccucuuuGACGAgCg -3' miRNA: 3'- gGCGCUUGCCau-CGcuuau--CUGCUgG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 66457 | 0.66 | 0.972916 |
Target: 5'- gCCGuCGAACucggugacgcuGGUGGCG-GUGGucuCGAUCa -3' miRNA: 3'- -GGC-GCUUG-----------CCAUCGCuUAUCu--GCUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 6548 | 0.66 | 0.972916 |
Target: 5'- -gGCGAGCGGUgaagcugugcgcGGCGuGUcAGGCcGCCu -3' miRNA: 3'- ggCGCUUGCCA------------UCGCuUA-UCUGcUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 63727 | 0.66 | 0.969891 |
Target: 5'- cCUGCGcAGcCGGUAGCGAcaucucGGCGGgCa -3' miRNA: 3'- -GGCGC-UU-GCCAUCGCUuau---CUGCUgG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 64017 | 0.66 | 0.969891 |
Target: 5'- aCCGCGccCGaGgcGCGGcgAGGCcgguGACCg -3' miRNA: 3'- -GGCGCuuGC-CauCGCUuaUCUG----CUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 54512 | 0.66 | 0.969891 |
Target: 5'- gCgGCGAuguGgGGUGGCGggUcGAguaucCGGCCc -3' miRNA: 3'- -GgCGCU---UgCCAUCGCuuAuCU-----GCUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 6166 | 0.66 | 0.969891 |
Target: 5'- gCGcCGAACGGUcgaGGUGAccaAUGGGUGAUCu -3' miRNA: 3'- gGC-GCUUGCCA---UCGCU---UAUCUGCUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 47789 | 0.66 | 0.966632 |
Target: 5'- aUCGCGcAGCGGUccgacGGUGAGUcgcgAGACcucgcgGGCCa -3' miRNA: 3'- -GGCGC-UUGCCA-----UCGCUUA----UCUG------CUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 43979 | 0.66 | 0.966632 |
Target: 5'- aCCGaGGGCGGU-GCcGAUGGACG-CUg -3' miRNA: 3'- -GGCgCUUGCCAuCGcUUAUCUGCuGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 29955 | 0.66 | 0.963131 |
Target: 5'- gUCGCGGcUGGUggGGCGGGcGG-UGACCg -3' miRNA: 3'- -GGCGCUuGCCA--UCGCUUaUCuGCUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 3093 | 0.66 | 0.963131 |
Target: 5'- gCCGCGcAGCGaugAGCG----GGCGGCCa -3' miRNA: 3'- -GGCGC-UUGCca-UCGCuuauCUGCUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 41590 | 0.66 | 0.962768 |
Target: 5'- gCGUGAGCGGUcGCGcgucgguguuucgGAUcgcgAGGCuGACCa -3' miRNA: 3'- gGCGCUUGCCAuCGC-------------UUA----UCUG-CUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 38319 | 0.67 | 0.959383 |
Target: 5'- uCUGUGucAUGGUGcccgaguugcGCGAGUAGACccuGACCu -3' miRNA: 3'- -GGCGCu-UGCCAU----------CGCUUAUCUG---CUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 46522 | 0.67 | 0.95538 |
Target: 5'- cCCGCGAGcCGGUcGgGGAUcgccgAGAucuggucgcCGACCg -3' miRNA: 3'- -GGCGCUU-GCCAuCgCUUA-----UCU---------GCUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 56483 | 0.67 | 0.95538 |
Target: 5'- gUCGCGAaaccguccucgACGGUGGCGAu--GAUucCCu -3' miRNA: 3'- -GGCGCU-----------UGCCAUCGCUuauCUGcuGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 49984 | 0.67 | 0.951118 |
Target: 5'- gCGCGcACGGaucGCGugcGGACGGCUg -3' miRNA: 3'- gGCGCuUGCCau-CGCuuaUCUGCUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 20451 | 0.67 | 0.951118 |
Target: 5'- aCGaCGAGCcggGGUGGCuc--GGAUGACCg -3' miRNA: 3'- gGC-GCUUG---CCAUCGcuuaUCUGCUGG- -5' |
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23216 | 3' | -50.5 | NC_005259.1 | + | 64897 | 0.67 | 0.951118 |
Target: 5'- gCCGCGugacacgagcGACGGcgAGCGugaGGAUGACg -3' miRNA: 3'- -GGCGC----------UUGCCa-UCGCuuaUCUGCUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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