Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23217 | 3' | -57.4 | NC_005259.1 | + | 54725 | 1.08 | 0.000863 |
Target: 5'- uACAGGACAUAGCCGAGGCUGCCGACAg -3' miRNA: 3'- -UGUCCUGUAUCGGCUCCGACGGCUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 15144 | 0.71 | 0.32792 |
Target: 5'- uACGGGGCcaccucgGUGGCCGAGGCcgagGCCauccucGACAc -3' miRNA: 3'- -UGUCCUG-------UAUCGGCUCCGa---CGG------CUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 24797 | 0.7 | 0.387043 |
Target: 5'- aGCAGGGCcaAGaCCGAGGUgccCCGGCAc -3' miRNA: 3'- -UGUCCUGuaUC-GGCUCCGac-GGCUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 68786 | 0.7 | 0.404919 |
Target: 5'- aGCAGGuCGUAcGCCGGGGCggUGUCGGu- -3' miRNA: 3'- -UGUCCuGUAU-CGGCUCCG--ACGGCUgu -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 60538 | 0.68 | 0.491358 |
Target: 5'- cCGGGACGcAGCCGcucgucGGCaugcccggUGCCGACGg -3' miRNA: 3'- uGUCCUGUaUCGGCu-----CCG--------ACGGCUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 52157 | 0.68 | 0.50048 |
Target: 5'- gGCAGG-CAgcGCCGAGGCgcucaUgcagcaggaccgcGCCGACAa -3' miRNA: 3'- -UGUCCuGUauCGGCUCCG-----A-------------CGGCUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 12501 | 0.68 | 0.51173 |
Target: 5'- uCAGG-CGUAGCCGcuGGGCcGCuCGGCu -3' miRNA: 3'- uGUCCuGUAUCGGC--UCCGaCG-GCUGu -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 33651 | 0.68 | 0.51173 |
Target: 5'- uCGGGAaaucAGCCGAGGgUGcCCGGCc -3' miRNA: 3'- uGUCCUgua-UCGGCUCCgAC-GGCUGu -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 6084 | 0.68 | 0.515847 |
Target: 5'- aACAGGACGcccucaacgccgagaUcGCCGAGGCcgagGCCGcCGc -3' miRNA: 3'- -UGUCCUGU---------------AuCGGCUCCGa---CGGCuGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 45813 | 0.68 | 0.522047 |
Target: 5'- -uGGGuGCGUAGCCGGGGaugaccaUGCCGuccGCAc -3' miRNA: 3'- ugUCC-UGUAUCGGCUCCg------ACGGC---UGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 23333 | 0.67 | 0.542913 |
Target: 5'- aGCcGGAUuacaucgGGCuCGAGGCUGCCuGCGa -3' miRNA: 3'- -UGuCCUGua-----UCG-GCUCCGACGGcUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 63576 | 0.67 | 0.596125 |
Target: 5'- aGCGGGAUAUAcGCCGccuGGCUGUucucguccuccaUGGCGg -3' miRNA: 3'- -UGUCCUGUAU-CGGCu--CCGACG------------GCUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 28007 | 0.67 | 0.596125 |
Target: 5'- cCGGGGCGgggucGCCGGGGUUGCgCG-CGu -3' miRNA: 3'- uGUCCUGUau---CGGCUCCGACG-GCuGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 58564 | 0.67 | 0.585392 |
Target: 5'- --cGGGCGguugGGCCGGaucgcggcgcGGCUGCCGGg- -3' miRNA: 3'- uguCCUGUa---UCGGCU----------CCGACGGCUgu -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 28648 | 0.67 | 0.585392 |
Target: 5'- ---cGACAccGCCGAGGCcGCCGuCAa -3' miRNA: 3'- ugucCUGUauCGGCUCCGaCGGCuGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 2160 | 0.67 | 0.585392 |
Target: 5'- cCAGcGGCGUcgAGCCGccGGUUGCCGAg- -3' miRNA: 3'- uGUC-CUGUA--UCGGCu-CCGACGGCUgu -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 5956 | 0.67 | 0.571497 |
Target: 5'- aGCGGGACuucugcgGGCCGAccaccaacuacacgGGCaGCCGAgAa -3' miRNA: 3'- -UGUCCUGua-----UCGGCU--------------CCGaCGGCUgU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 44980 | 0.67 | 0.552393 |
Target: 5'- -gAGGGCcgGugcgacuGCCGAGGCaccGCCGACc -3' miRNA: 3'- ugUCCUGuaU-------CGGCUCCGa--CGGCUGu -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 22791 | 0.66 | 0.606887 |
Target: 5'- uGCAGGuGCuca-CCGAGGgUGCCGAUu -3' miRNA: 3'- -UGUCC-UGuaucGGCUCCgACGGCUGu -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 50665 | 0.66 | 0.606887 |
Target: 5'- cAUAGGGCAccAGCgCGucGgUGCCGACGa -3' miRNA: 3'- -UGUCCUGUa-UCG-GCucCgACGGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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