Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23217 | 3' | -57.4 | NC_005259.1 | + | 10476 | 0.66 | 0.61767 |
Target: 5'- aGCGGGACgaucuuggggucGUGGUuguUGGGGUUGuCCGGCAc -3' miRNA: 3'- -UGUCCUG------------UAUCG---GCUCCGAC-GGCUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 27070 | 0.66 | 0.61767 |
Target: 5'- cACGGcGACAUcGCCGAcauucugcggcGGCUGCCa--- -3' miRNA: 3'- -UGUC-CUGUAuCGGCU-----------CCGACGGcugu -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 6210 | 0.66 | 0.628466 |
Target: 5'- aGCGGGGug-AGCCGccGC-GCCGACAc -3' miRNA: 3'- -UGUCCUguaUCGGCucCGaCGGCUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 59481 | 0.66 | 0.639264 |
Target: 5'- gGCAGGAUggcccgAUAGCCGAGaGUgucaccGcCCGGCGa -3' miRNA: 3'- -UGUCCUG------UAUCGGCUC-CGa-----C-GGCUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 46294 | 0.66 | 0.660833 |
Target: 5'- aGCGGGGCc--GCCGuGGCUGCguuguucgCGGCc -3' miRNA: 3'- -UGUCCUGuauCGGCuCCGACG--------GCUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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