Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23217 | 3' | -57.4 | NC_005259.1 | + | 27070 | 0.66 | 0.61767 |
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Target: 5'- cACGGcGACAUcGCCGAcauucugcggcGGCUGCCa--- -3' miRNA: 3'- -UGUC-CUGUAuCGGCU-----------CCGACGGcugu -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 10476 | 0.66 | 0.61767 |
|
Target: 5'- aGCGGGACgaucuuggggucGUGGUuguUGGGGUUGuCCGGCAc -3' miRNA: 3'- -UGUCCUG------------UAUCG---GCUCCGAC-GGCUGU- -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 50665 | 0.66 | 0.606887 |
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Target: 5'- cAUAGGGCAccAGCgCGucGgUGCCGACGa -3' miRNA: 3'- -UGUCCUGUa-UCG-GCucCgACGGCUGU- -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 22791 | 0.66 | 0.606887 |
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Target: 5'- uGCAGGuGCuca-CCGAGGgUGCCGAUu -3' miRNA: 3'- -UGUCC-UGuaucGGCUCCgACGGCUGu -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 46294 | 0.66 | 0.660833 |
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Target: 5'- aGCGGGGCc--GCCGuGGCUGCguuguucgCGGCc -3' miRNA: 3'- -UGUCCUGuauCGGCuCCGACG--------GCUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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