Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23217 | 3' | -57.4 | NC_005259.1 | + | 60538 | 0.68 | 0.491358 |
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Target: 5'- cCGGGACGcAGCCGcucgucGGCaugcccggUGCCGACGg -3' miRNA: 3'- uGUCCUGUaUCGGCu-----CCG--------ACGGCUGU- -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 68786 | 0.7 | 0.404919 |
|
Target: 5'- aGCAGGuCGUAcGCCGGGGCggUGUCGGu- -3' miRNA: 3'- -UGUCCuGUAU-CGGCUCCG--ACGGCUgu -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 24797 | 0.7 | 0.387043 |
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Target: 5'- aGCAGGGCcaAGaCCGAGGUgccCCGGCAc -3' miRNA: 3'- -UGUCCUGuaUC-GGCUCCGac-GGCUGU- -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 15144 | 0.71 | 0.32792 |
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Target: 5'- uACGGGGCcaccucgGUGGCCGAGGCcgagGCCauccucGACAc -3' miRNA: 3'- -UGUCCUG-------UAUCGGCUCCGa---CGG------CUGU- -5' |
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| 23217 | 3' | -57.4 | NC_005259.1 | + | 54725 | 1.08 | 0.000863 |
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Target: 5'- uACAGGACAUAGCCGAGGCUGCCGACAg -3' miRNA: 3'- -UGUCCUGUAUCGGCUCCGACGGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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