Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23217 | 3' | -57.4 | NC_005259.1 | + | 33651 | 0.68 | 0.51173 |
Target: 5'- uCGGGAaaucAGCCGAGGgUGcCCGGCc -3' miRNA: 3'- uGUCCUgua-UCGGCUCCgAC-GGCUGu -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 6084 | 0.68 | 0.515847 |
Target: 5'- aACAGGACGcccucaacgccgagaUcGCCGAGGCcgagGCCGcCGc -3' miRNA: 3'- -UGUCCUGU---------------AuCGGCUCCGa---CGGCuGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 45813 | 0.68 | 0.522047 |
Target: 5'- -uGGGuGCGUAGCCGGGGaugaccaUGCCGuccGCAc -3' miRNA: 3'- ugUCC-UGUAUCGGCUCCg------ACGGC---UGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 23333 | 0.67 | 0.542913 |
Target: 5'- aGCcGGAUuacaucgGGCuCGAGGCUGCCuGCGa -3' miRNA: 3'- -UGuCCUGua-----UCG-GCUCCGACGGcUGU- -5' |
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23217 | 3' | -57.4 | NC_005259.1 | + | 46294 | 0.66 | 0.660833 |
Target: 5'- aGCGGGGCc--GCCGuGGCUGCguuguucgCGGCc -3' miRNA: 3'- -UGUCCUGuauCGGCuCCGACG--------GCUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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