Results 21 - 33 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23221 | 5' | -57.1 | NC_005259.1 | + | 45954 | 0.66 | 0.67909 |
Target: 5'- cAGCACguuGCCGACGuugccGAgcuUGGCGAGUg -3' miRNA: 3'- -UCGUG---UGGCUGCu----CUag-GCCGCUCAa -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 46972 | 0.66 | 0.646913 |
Target: 5'- uGGcCACGCCGcCGAuGAUCgGGcCGAGg- -3' miRNA: 3'- -UC-GUGUGGCuGCU-CUAGgCC-GCUCaa -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 51312 | 0.67 | 0.571799 |
Target: 5'- cAGUGCGCCGAgaaucucuuugaCGAGGaugCCGGUGAGc- -3' miRNA: 3'- -UCGUGUGGCU------------GCUCUa--GGCCGCUCaa -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 52801 | 1.05 | 0.00171 |
Target: 5'- cAGCACACCGACGAGAUCCGGCGAGUUu -3' miRNA: 3'- -UCGUGUGGCUGCUCUAGGCCGCUCAA- -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 58740 | 0.71 | 0.385128 |
Target: 5'- uGGCaccgGCACCGGCGAaGUUCGGCGAa-- -3' miRNA: 3'- -UCG----UGUGGCUGCUcUAGGCCGCUcaa -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 61743 | 0.69 | 0.509116 |
Target: 5'- uGUGCGuCCGGCGAGAUCUGuGCGGc-- -3' miRNA: 3'- uCGUGU-GGCUGCUCUAGGC-CGCUcaa -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 63616 | 0.67 | 0.62538 |
Target: 5'- cGGC-CACCGACGccugauAGAUCaucguGGCGAGc- -3' miRNA: 3'- -UCGuGUGGCUGC------UCUAGg----CCGCUCaa -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 63850 | 0.7 | 0.439943 |
Target: 5'- aAGCGCguACCGGCGAGGUggCCGGCuGGc- -3' miRNA: 3'- -UCGUG--UGGCUGCUCUA--GGCCGcUCaa -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 64593 | 0.73 | 0.269124 |
Target: 5'- cAGCACACCaACGAGGUgacugaCGGCGAGc- -3' miRNA: 3'- -UCGUGUGGcUGCUCUAg-----GCCGCUCaa -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 65185 | 0.71 | 0.351183 |
Target: 5'- aGGCGCuggUCGAUcAGAUCCGGUGGGUa -3' miRNA: 3'- -UCGUGu--GGCUGcUCUAGGCCGCUCAa -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 65698 | 0.72 | 0.343035 |
Target: 5'- gAGCGgGCCGAguaggaCGAGGUCacgcccaaGGCGAGUUg -3' miRNA: 3'- -UCGUgUGGCU------GCUCUAGg-------CCGCUCAA- -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 67891 | 0.66 | 0.646913 |
Target: 5'- cGCGCAUCGuuCGgcuguGGGUCuCGGCGAGg- -3' miRNA: 3'- uCGUGUGGCu-GC-----UCUAG-GCCGCUCaa -5' |
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23221 | 5' | -57.1 | NC_005259.1 | + | 68880 | 0.73 | 0.297039 |
Target: 5'- gGGCGCGCCGAUGAGGUagaugaGGCG-GUg -3' miRNA: 3'- -UCGUGUGGCUGCUCUAgg----CCGCuCAa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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