Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23223 | 3' | -56.3 | NC_005259.1 | + | 50706 | 0.69 | 0.571831 |
Target: 5'- -uCGUCGcgCUUGAgCCGGGUGCugGc -3' miRNA: 3'- cuGCGGCuaGAGCU-GGCCUACGugCu -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 43418 | 0.66 | 0.759887 |
Target: 5'- cGGCGgCGGUuuccucgCUCGACUGGcUGCGCu- -3' miRNA: 3'- -CUGCgGCUA-------GAGCUGGCCuACGUGcu -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 67357 | 0.66 | 0.720249 |
Target: 5'- -uCGCCGAcCUCGcGCCGGAUcUugGGg -3' miRNA: 3'- cuGCGGCUaGAGC-UGGCCUAcGugCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 50213 | 0.66 | 0.720249 |
Target: 5'- aGCGCCGccucgucaAUCUCGAUCcGA-GCGCGGg -3' miRNA: 3'- cUGCGGC--------UAGAGCUGGcCUaCGUGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 39837 | 0.66 | 0.720249 |
Target: 5'- cGCGuuGGUCggCGACaGGAUGC-CGGu -3' miRNA: 3'- cUGCggCUAGa-GCUGgCCUACGuGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 64035 | 0.66 | 0.71714 |
Target: 5'- cGAgGCCGGugaccggaucuuucUCUUG-CCGGAUcaGCACGGc -3' miRNA: 3'- -CUgCGGCU--------------AGAGCuGGCCUA--CGUGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 38298 | 0.66 | 0.709858 |
Target: 5'- cGGCGCUGAUCgUCgGACCGaucugugucauGGUGCcCGAg -3' miRNA: 3'- -CUGCGGCUAG-AG-CUGGC-----------CUACGuGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 42882 | 0.67 | 0.699396 |
Target: 5'- --gGUCGGUCUCGGUgGGGUugaGCACGAg -3' miRNA: 3'- cugCGGCUAGAGCUGgCCUA---CGUGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 31890 | 0.67 | 0.688873 |
Target: 5'- -uCGCCGGUCUCaaGGCCcg--GCGCGAg -3' miRNA: 3'- cuGCGGCUAGAG--CUGGccuaCGUGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 24273 | 0.67 | 0.667681 |
Target: 5'- cGugGCUGGaagcgCUCGACCucgGGAuggugcugcUGCGCGAu -3' miRNA: 3'- -CugCGGCUa----GAGCUGG---CCU---------ACGUGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 65987 | 0.68 | 0.624997 |
Target: 5'- cGCGCCGAgcagccgCUCGACgUGG-UGCGCa- -3' miRNA: 3'- cUGCGGCUa------GAGCUG-GCCuACGUGcu -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 51995 | 1.09 | 0.001161 |
Target: 5'- cGACGCCGAUCUCGACCGGAUGCACGAg -3' miRNA: 3'- -CUGCGGCUAGAGCUGGCCUACGUGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 50412 | 0.69 | 0.540425 |
Target: 5'- cGCGCCGGUgcCUUGACCGGcucggGCAgCGGu -3' miRNA: 3'- cUGCGGCUA--GAGCUGGCCua---CGU-GCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 50836 | 0.69 | 0.540425 |
Target: 5'- cGACuCCGAUCUCGACaGGGgccaCGCGAg -3' miRNA: 3'- -CUGcGGCUAGAGCUGgCCUac--GUGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 17904 | 0.71 | 0.431542 |
Target: 5'- cGCGCCGAUg-CGugUGGAUGCcCGGu -3' miRNA: 3'- cUGCGGCUAgaGCugGCCUACGuGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 66922 | 0.71 | 0.422275 |
Target: 5'- aGCGCgCGGUgCUCGACCGGGgugaGCGgGAc -3' miRNA: 3'- cUGCG-GCUA-GAGCUGGCCUa---CGUgCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 68132 | 0.71 | 0.422275 |
Target: 5'- aGCGgCGAgCUCGGCaGGAUGCGCGu -3' miRNA: 3'- cUGCgGCUaGAGCUGgCCUACGUGCu -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 14722 | 0.74 | 0.32104 |
Target: 5'- cACGCCGcgCUCGucGCCGaGAUGUACGc -3' miRNA: 3'- cUGCGGCuaGAGC--UGGC-CUACGUGCu -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 8269 | 0.74 | 0.298745 |
Target: 5'- cGACGuaGGUgUUGuACCGGGUGCGCGGg -3' miRNA: 3'- -CUGCggCUAgAGC-UGGCCUACGUGCU- -5' |
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23223 | 3' | -56.3 | NC_005259.1 | + | 51511 | 0.75 | 0.263629 |
Target: 5'- -cCGCCGAUCUCGACgCGcGugaccucGUGCACGGg -3' miRNA: 3'- cuGCGGCUAGAGCUG-GC-C-------UACGUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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