Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23223 | 3' | -56.3 | NC_005259.1 | + | 66922 | 0.71 | 0.422275 |
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Target: 5'- aGCGCgCGGUgCUCGACCGGGgugaGCGgGAc -3' miRNA: 3'- cUGCG-GCUA-GAGCUGGCCUa---CGUgCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 68132 | 0.71 | 0.422275 |
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Target: 5'- aGCGgCGAgCUCGGCaGGAUGCGCGu -3' miRNA: 3'- cUGCgGCUaGAGCUGgCCUACGUGCu -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 14722 | 0.74 | 0.32104 |
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Target: 5'- cACGCCGcgCUCGucGCCGaGAUGUACGc -3' miRNA: 3'- cUGCGGCuaGAGC--UGGC-CUACGUGCu -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 8269 | 0.74 | 0.298745 |
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Target: 5'- cGACGuaGGUgUUGuACCGGGUGCGCGGg -3' miRNA: 3'- -CUGCggCUAgAGC-UGGCCUACGUGCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 51511 | 0.75 | 0.263629 |
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Target: 5'- -cCGCCGAUCUCGACgCGcGugaccucGUGCACGGg -3' miRNA: 3'- cuGCGGCUAGAGCUG-GC-C-------UACGUGCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 300 | 0.85 | 0.055344 |
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Target: 5'- cGCGCUGAUCUCGACCGGggGUGCGu -3' miRNA: 3'- cUGCGGCUAGAGCUGGCCuaCGUGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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