Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23223 | 3' | -56.3 | NC_005259.1 | + | 17904 | 0.71 | 0.431542 |
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Target: 5'- cGCGCCGAUg-CGugUGGAUGCcCGGu -3' miRNA: 3'- cUGCGGCUAgaGCugGCCUACGuGCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 50836 | 0.69 | 0.540425 |
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Target: 5'- cGACuCCGAUCUCGACaGGGgccaCGCGAg -3' miRNA: 3'- -CUGcGGCUAGAGCUGgCCUac--GUGCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 50412 | 0.69 | 0.540425 |
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Target: 5'- cGCGCCGGUgcCUUGACCGGcucggGCAgCGGu -3' miRNA: 3'- cUGCGGCUA--GAGCUGGCCua---CGU-GCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 50706 | 0.69 | 0.571831 |
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Target: 5'- -uCGUCGcgCUUGAgCCGGGUGCugGc -3' miRNA: 3'- cuGCGGCuaGAGCU-GGCCUACGugCu -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 65987 | 0.68 | 0.624997 |
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Target: 5'- cGCGCCGAgcagccgCUCGACgUGG-UGCGCa- -3' miRNA: 3'- cUGCGGCUa------GAGCUG-GCCuACGUGcu -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 43418 | 0.66 | 0.759887 |
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Target: 5'- cGGCGgCGGUuuccucgCUCGACUGGcUGCGCu- -3' miRNA: 3'- -CUGCgGCUA-------GAGCUGGCCuACGUGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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