Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23223 | 3' | -56.3 | NC_005259.1 | + | 51995 | 1.09 | 0.001161 |
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Target: 5'- cGACGCCGAUCUCGACCGGAUGCACGAg -3' miRNA: 3'- -CUGCGGCUAGAGCUGGCCUACGUGCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 64035 | 0.66 | 0.71714 |
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Target: 5'- cGAgGCCGGugaccggaucuuucUCUUG-CCGGAUcaGCACGGc -3' miRNA: 3'- -CUgCGGCU--------------AGAGCuGGCCUA--CGUGCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 65987 | 0.68 | 0.624997 |
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Target: 5'- cGCGCCGAgcagccgCUCGACgUGG-UGCGCa- -3' miRNA: 3'- cUGCGGCUa------GAGCUG-GCCuACGUGcu -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 66922 | 0.71 | 0.422275 |
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Target: 5'- aGCGCgCGGUgCUCGACCGGGgugaGCGgGAc -3' miRNA: 3'- cUGCG-GCUA-GAGCUGGCCUa---CGUgCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 67357 | 0.66 | 0.720249 |
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Target: 5'- -uCGCCGAcCUCGcGCCGGAUcUugGGg -3' miRNA: 3'- cuGCGGCUaGAGC-UGGCCUAcGugCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 68132 | 0.71 | 0.422275 |
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Target: 5'- aGCGgCGAgCUCGGCaGGAUGCGCGu -3' miRNA: 3'- cUGCgGCUaGAGCUGgCCUACGUGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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