Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23223 | 3' | -56.3 | NC_005259.1 | + | 45520 | 0.66 | 0.740772 |
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Target: 5'- cGCGCCGGUgUUGGCCGccgugGCuGCGAg -3' miRNA: 3'- cUGCGGCUAgAGCUGGCcua--CG-UGCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 6011 | 0.66 | 0.740772 |
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Target: 5'- cGCGUCGAgaucgagCUCGAUgagCGGAUGUucaGCGAc -3' miRNA: 3'- cUGCGGCUa------GAGCUG---GCCUACG---UGCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 11589 | 0.66 | 0.750883 |
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Target: 5'- --aGCgGG-CUUGGCCGGAguuUGCugGAa -3' miRNA: 3'- cugCGgCUaGAGCUGGCCU---ACGugCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 49985 | 0.66 | 0.750883 |
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Target: 5'- cGCGCacgGAUCgCGugCGGAcggcugcccucgUGCACGGg -3' miRNA: 3'- cUGCGg--CUAGaGCugGCCU------------ACGUGCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 51315 | 0.66 | 0.750883 |
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Target: 5'- uGCGCCGAgaaUCUCuuuGACgaGGAUGC-CGGu -3' miRNA: 3'- cUGCGGCU---AGAG---CUGg-CCUACGuGCU- -5' |
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| 23223 | 3' | -56.3 | NC_005259.1 | + | 43418 | 0.66 | 0.759887 |
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Target: 5'- cGGCGgCGGUuuccucgCUCGACUGGcUGCGCu- -3' miRNA: 3'- -CUGCgGCUA-------GAGCUGGCCuACGUGcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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