Results 1 - 20 of 51 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23224 | 3' | -59.5 | NC_005259.1 | + | 25906 | 0.66 | 0.512927 |
Target: 5'- -aGGaugGCCGCcUGAGCCGC-CgCCGCc -3' miRNA: 3'- uaUCg--CGGCGuACUCGGCGuG-GGUGu -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 38948 | 0.66 | 0.512927 |
Target: 5'- -cGGUGCCGCu---GCCGCcgagaGCCUGCAc -3' miRNA: 3'- uaUCGCGGCGuacuCGGCG-----UGGGUGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 4641 | 0.66 | 0.512927 |
Target: 5'- gAUAGUGcCCGCGca--CCGCGCCUACGg -3' miRNA: 3'- -UAUCGC-GGCGUacucGGCGUGGGUGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 44687 | 0.66 | 0.502729 |
Target: 5'- cGUGGC-CCGCAuaUGGcCCGCGuCCCACc -3' miRNA: 3'- -UAUCGcGGCGU--ACUcGGCGU-GGGUGu -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 8775 | 0.66 | 0.502729 |
Target: 5'- -cAGCGCgGCGgugaUGAGCUcgGCCCGCu -3' miRNA: 3'- uaUCGCGgCGU----ACUCGGcgUGGGUGu -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 18791 | 0.66 | 0.502729 |
Target: 5'- -aAGCgGUgGCAggGAcGCCGCACCCuCAa -3' miRNA: 3'- uaUCG-CGgCGUa-CU-CGGCGUGGGuGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 1354 | 0.66 | 0.502729 |
Target: 5'- -cGGUGCCuacUGGGCCGCucgccgcgccGCCCGCGa -3' miRNA: 3'- uaUCGCGGcguACUCGGCG----------UGGGUGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 50150 | 0.66 | 0.502729 |
Target: 5'- uUGGCGauccggCGCGUGAGCgaGCgguggaauGCCCGCAg -3' miRNA: 3'- uAUCGCg-----GCGUACUCGg-CG--------UGGGUGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 38746 | 0.66 | 0.49262 |
Target: 5'- gGUGGCccGCCGCAggccggUGAGCUGCGCgaUCAUg -3' miRNA: 3'- -UAUCG--CGGCGU------ACUCGGCGUG--GGUGu -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 31799 | 0.66 | 0.489605 |
Target: 5'- --cGCGUCGuCAUcGAGCucgaccgaccccacCGCGCCCGCGu -3' miRNA: 3'- uauCGCGGC-GUA-CUCG--------------GCGUGGGUGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 45344 | 0.66 | 0.482605 |
Target: 5'- --uGCGCCGCcUGGGCCGcCGCgUAg- -3' miRNA: 3'- uauCGCGGCGuACUCGGC-GUGgGUgu -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 14874 | 0.66 | 0.482605 |
Target: 5'- --cGCGCUGCccGAGCCGcCGCCg--- -3' miRNA: 3'- uauCGCGGCGuaCUCGGC-GUGGgugu -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 34688 | 0.66 | 0.47269 |
Target: 5'- uUGGCGCUGCGgaUGAuGCCGaccauCugCCGCGc -3' miRNA: 3'- uAUCGCGGCGU--ACU-CGGC-----GugGGUGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 26045 | 0.66 | 0.47269 |
Target: 5'- -cAGCGCCGCcgGGuuggccauGCCGcCGCCgaGCAg -3' miRNA: 3'- uaUCGCGGCGuaCU--------CGGC-GUGGg-UGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 27444 | 0.67 | 0.462878 |
Target: 5'- --cGCGUCGCAUGAGCuCGU-CgCGCGg -3' miRNA: 3'- uauCGCGGCGUACUCG-GCGuGgGUGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 24813 | 0.67 | 0.462878 |
Target: 5'- -aGGUGCCccgGCAc--GCCGUGCCCGCAc -3' miRNA: 3'- uaUCGCGG---CGUacuCGGCGUGGGUGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 64689 | 0.67 | 0.453175 |
Target: 5'- --cGCGCCGCc-GAGCgaUACCCACAu -3' miRNA: 3'- uauCGCGGCGuaCUCGgcGUGGGUGU- -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 52191 | 0.67 | 0.44263 |
Target: 5'- --cGCGCCGacaacgcacggcuCAUGGGCaccgcguucCGCGCCCGCu -3' miRNA: 3'- uauCGCGGC-------------GUACUCG---------GCGUGGGUGu -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 28955 | 0.67 | 0.44263 |
Target: 5'- --uGCGCCGCAucgccgaggucggUGccgaGGCCGCgagcGCCCGCu -3' miRNA: 3'- uauCGCGGCGU-------------AC----UCGGCG----UGGGUGu -5' |
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23224 | 3' | -59.5 | NC_005259.1 | + | 46902 | 0.67 | 0.441678 |
Target: 5'- -gAGCGCCcgccgagcaguccGCGUGcGCCGCcgagcuuGCCCGCc -3' miRNA: 3'- uaUCGCGG-------------CGUACuCGGCG-------UGGGUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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