Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23238 | 5' | -56 | NC_005259.1 | + | 1290 | 0.66 | 0.750883 |
Target: 5'- aCGAcCCCGagGUCGagcucuacGCGGUCGuGAGCGc -3' miRNA: 3'- -GCUaGGGCg-UAGC--------CGCCAGCuCUUGU- -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 28219 | 0.69 | 0.582402 |
Target: 5'- aCGGUCgugCCGCcaccGUCGGCGG-CGGGGugGu -3' miRNA: 3'- -GCUAG---GGCG----UAGCCGCCaGCUCUugU- -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 33312 | 0.79 | 0.153603 |
Target: 5'- uCGAUCCCGcCGUCGGCGGUCacgcGGAAg- -3' miRNA: 3'- -GCUAGGGC-GUAGCCGCCAGc---UCUUgu -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 38871 | 0.67 | 0.678298 |
Target: 5'- aCGcgCCCGCAaUGGCGGUguUGAcGACGc -3' miRNA: 3'- -GCuaGGGCGUaGCCGCCA--GCUcUUGU- -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 42147 | 0.7 | 0.550836 |
Target: 5'- uCGcgCCCGCGUUGGUGcacgCGAcGAGCAg -3' miRNA: 3'- -GCuaGGGCGUAGCCGCca--GCU-CUUGU- -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 44770 | 1.09 | 0.001304 |
Target: 5'- cCGAUCCCGCAUCGGCGGUCGAGAACAg -3' miRNA: 3'- -GCUAGGGCGUAGCCGCCAGCUCUUGU- -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 46517 | 0.72 | 0.422275 |
Target: 5'- aCGAUCCCGCga-GcCGGUCGGGGAUc -3' miRNA: 3'- -GCUAGGGCGuagCcGCCAGCUCUUGu -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 49714 | 0.79 | 0.153603 |
Target: 5'- aGAUCCgGCAgauauUCGGCGGgcggCGAGAGCu -3' miRNA: 3'- gCUAGGgCGU-----AGCCGCCa---GCUCUUGu -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 50262 | 0.67 | 0.709858 |
Target: 5'- aCGGgcuaCUCGCugucGUCGGUGG-CGGGAACGu -3' miRNA: 3'- -GCUa---GGGCG----UAGCCGCCaGCUCUUGU- -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 56927 | 0.66 | 0.770755 |
Target: 5'- ---gCCCGCGUCGGUgagcaugcGGUUGGcGAACu -3' miRNA: 3'- gcuaGGGCGUAGCCG--------CCAGCU-CUUGu -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 58921 | 0.7 | 0.509621 |
Target: 5'- gCGGUCuuGuCGUCGGCGGgcgCGGuGGCAg -3' miRNA: 3'- -GCUAGggC-GUAGCCGCCa--GCUcUUGU- -5' |
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23238 | 5' | -56 | NC_005259.1 | + | 61197 | 0.66 | 0.779526 |
Target: 5'- cCGGUCUgcuugcaccacauCGCAggaagCGGCGGUgugcgcacCGAGAACu -3' miRNA: 3'- -GCUAGG-------------GCGUa----GCCGCCA--------GCUCUUGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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