Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23240 | 5' | -57.5 | NC_005259.1 | + | 6190 | 0.69 | 0.465245 |
Target: 5'- uGGGugaUCUGaGCAGCgugAGCGGggUGAGCCg -3' miRNA: 3'- -UCC---AGAC-CGUCG---UCGCUgaACUCGGg -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 68120 | 0.66 | 0.643094 |
Target: 5'- cGGGUCgag-AGCAGCGGC--GAGCUCg -3' miRNA: 3'- -UCCAGaccgUCGUCGCUGaaCUCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 36494 | 0.66 | 0.62153 |
Target: 5'- cGGUCgacgGGCAGCucuGGCGugucCUUGAaccaCCCg -3' miRNA: 3'- uCCAGa---CCGUCG---UCGCu---GAACUc---GGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 39762 | 0.67 | 0.578577 |
Target: 5'- -----cGGCAGCAGCGGC---AGCCUg -3' miRNA: 3'- uccagaCCGUCGUCGCUGaacUCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 36429 | 0.67 | 0.57218 |
Target: 5'- uGGUCUGcGCAGCGucgccgaggucgaccGCGuCgagggUGAGCaCCg -3' miRNA: 3'- uCCAGAC-CGUCGU---------------CGCuGa----ACUCG-GG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 48060 | 0.67 | 0.564739 |
Target: 5'- cGG-CgGGUAGCgccgccgcgcccugAGCGAUggugUUGAGCCCg -3' miRNA: 3'- uCCaGaCCGUCG--------------UCGCUG----AACUCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 45188 | 0.67 | 0.556266 |
Target: 5'- gAGGUCaGcGCGGUgaugacgGGCGAgUucgcguUGAGCCCg -3' miRNA: 3'- -UCCAGaC-CGUCG-------UCGCUgA------ACUCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 10340 | 0.68 | 0.536298 |
Target: 5'- cGGG-CUcGGCGGCAGCGgGCUcgacaucGGGCUCg -3' miRNA: 3'- -UCCaGA-CCGUCGUCGC-UGAa------CUCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 27136 | 0.68 | 0.536298 |
Target: 5'- cGGUUugagGGCGGCuGCgGugUUGGGgCCa -3' miRNA: 3'- uCCAGa---CCGUCGuCG-CugAACUCgGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 8757 | 0.68 | 0.525889 |
Target: 5'- gAGGUCgaggcccuUGGcCAGC-GCGGCggugaUGAGCUCg -3' miRNA: 3'- -UCCAG--------ACC-GUCGuCGCUGa----ACUCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 43113 | 1.1 | 0.000551 |
Target: 5'- cAGGUCUGGCAGCAGCGACUUGAGCCCc -3' miRNA: 3'- -UCCAGACCGUCGUCGCUGAACUCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 2151 | 0.7 | 0.39049 |
Target: 5'- cGGUgCUGGcCAGCGGCGuc--GAGCCg -3' miRNA: 3'- uCCA-GACC-GUCGUCGCugaaCUCGGg -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 55331 | 0.71 | 0.356171 |
Target: 5'- uGGUCUuggcGGCAGCGGUGGCgau-GCCg -3' miRNA: 3'- uCCAGA----CCGUCGUCGCUGaacuCGGg -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 67787 | 0.73 | 0.272415 |
Target: 5'- cGGUgaccuugUUGGCAccGCGuGCGAgCUUGAGCCCg -3' miRNA: 3'- uCCA-------GACCGU--CGU-CGCU-GAACUCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 27397 | 0.75 | 0.200982 |
Target: 5'- cGGUgUcGGCAGCGGCGGCagcucgGuGCCCg -3' miRNA: 3'- uCCAgA-CCGUCGUCGCUGaa----CuCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 58968 | 0.75 | 0.195786 |
Target: 5'- cAGGUCgGGCAGCucgucGGCGACggcGAGCUUg -3' miRNA: 3'- -UCCAGaCCGUCG-----UCGCUGaa-CUCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 59306 | 0.76 | 0.176163 |
Target: 5'- -uGUCgUGGCGGCAGCGGC--GAGCUCa -3' miRNA: 3'- ucCAG-ACCGUCGUCGCUGaaCUCGGG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 36388 | 0.76 | 0.167022 |
Target: 5'- gAGG-UUGGcCAGCgcgAGCGACUUGAGCgCCu -3' miRNA: 3'- -UCCaGACC-GUCG---UCGCUGAACUCG-GG- -5' |
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23240 | 5' | -57.5 | NC_005259.1 | + | 11509 | 0.81 | 0.075131 |
Target: 5'- uAGGUCaggccauccucGGCGGCAGCGAuCUUGAGCCg -3' miRNA: 3'- -UCCAGa----------CCGUCGUCGCU-GAACUCGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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