Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23243 | 5' | -55.2 | NC_005259.1 | + | 42446 | 1.08 | 0.001566 |
Target: 5'- gUGUUCGGCGUCCAACAGUCGCCGACUa -3' miRNA: 3'- -ACAAGCCGCAGGUUGUCAGCGGCUGA- -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 23304 | 0.72 | 0.457821 |
Target: 5'- --aUCGGgGUCCGGCGcggucgguGUCGCCGAg- -3' miRNA: 3'- acaAGCCgCAGGUUGU--------CAGCGGCUga -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 42504 | 0.7 | 0.549574 |
Target: 5'- cGUcCGGCGguguggCCAGCAGcCGuCCGGCc -3' miRNA: 3'- aCAaGCCGCa-----GGUUGUCaGC-GGCUGa -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 27933 | 0.69 | 0.592241 |
Target: 5'- gGUUCGGCGacCCAGC--UCGCCGGu- -3' miRNA: 3'- aCAAGCCGCa-GGUUGucAGCGGCUga -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 60050 | 0.69 | 0.612721 |
Target: 5'- cGUUCGGgGUCUucucGggcucaggcucggGCGGcUCGCCGACUu -3' miRNA: 3'- aCAAGCCgCAGG----U-------------UGUC-AGCGGCUGA- -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 60738 | 0.68 | 0.657032 |
Target: 5'- cGUgUCGGgGUCCAuuugcaguGCAGUCgGCuCGGCg -3' miRNA: 3'- aCA-AGCCgCAGGU--------UGUCAG-CG-GCUGa -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 2156 | 0.67 | 0.710487 |
Target: 5'- cUGgccagCGGCGUCgAGCcgccGGUUGCCGAg- -3' miRNA: 3'- -ACaa---GCCGCAGgUUG----UCAGCGGCUga -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 61605 | 0.67 | 0.710487 |
Target: 5'- gUGgggUCGGCGgggaaCAGCAGaccggcacCGCCGACg -3' miRNA: 3'- -ACa--AGCCGCag---GUUGUCa-------GCGGCUGa -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 20651 | 0.67 | 0.740724 |
Target: 5'- cGUUCGGCGgcaccggggCCGAggacgccaugaugUGGUCGCaCGGCa -3' miRNA: 3'- aCAAGCCGCa--------GGUU-------------GUCAGCG-GCUGa -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 34485 | 0.66 | 0.75906 |
Target: 5'- ---gUGGUGUCCcccgccgacgcgacGACGauGUCGCCGACa -3' miRNA: 3'- acaaGCCGCAGG--------------UUGU--CAGCGGCUGa -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 53241 | 0.66 | 0.772055 |
Target: 5'- gGUUCGcGcCG-CCGACAccGUCGCCGccGCUg -3' miRNA: 3'- aCAAGC-C-GCaGGUUGU--CAGCGGC--UGA- -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 42387 | 0.66 | 0.781892 |
Target: 5'- cGggCaGGCcgGUCCAugAGUCGUCGuACUc -3' miRNA: 3'- aCaaG-CCG--CAGGUugUCAGCGGC-UGA- -5' |
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23243 | 5' | -55.2 | NC_005259.1 | + | 35262 | 0.66 | 0.791578 |
Target: 5'- uUGUUCGcGCccgCCGcgccGgGGUCGCCGAUg -3' miRNA: 3'- -ACAAGC-CGca-GGU----UgUCAGCGGCUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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