Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23248 | 5' | -58.1 | NC_005259.1 | + | 48541 | 0.67 | 0.546778 |
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Target: 5'- -gGCgCUGUcgCCGGUCUUGUUGuacGCGCg -3' miRNA: 3'- uaUGgGACG--GGUCGGAGCAACu--CGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 4281 | 0.67 | 0.550989 |
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Target: 5'- cUGCgCUGCCacgagcacggaccggCGGCCcugCGaUGAGCGCg -3' miRNA: 3'- uAUGgGACGG---------------GUCGGa--GCaACUCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 29525 | 0.66 | 0.557323 |
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Target: 5'- aAUGCCCU-CCCGGUCUCaucagcaaacUUGAcGCGCu -3' miRNA: 3'- -UAUGGGAcGGGUCGGAGc---------AACU-CGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 58642 | 0.66 | 0.566863 |
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Target: 5'- aGUGCCCaUGCCCucgcgguAGCC-CGgugcaccgGGGUGCa -3' miRNA: 3'- -UAUGGG-ACGGG-------UCGGaGCaa------CUCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 4328 | 0.66 | 0.567925 |
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Target: 5'- -aACCUcgUGCCgGaCCUCGUcgcggugaUGAGCGCc -3' miRNA: 3'- uaUGGG--ACGGgUcGGAGCA--------ACUCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 24317 | 0.66 | 0.589272 |
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Target: 5'- cUACCCgacaugGUCCGGCCUCac---GCGCu -3' miRNA: 3'- uAUGGGa-----CGGGUCGGAGcaacuCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 49669 | 0.66 | 0.600001 |
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Target: 5'- -cGCgCCUGCUCGGCCUgGUaGucggccucGCGCu -3' miRNA: 3'- uaUG-GGACGGGUCGGAgCAaCu-------CGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 3292 | 0.66 | 0.610757 |
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Target: 5'- -cGCCUcGCCgGucCCUCGgcGAGCGCu -3' miRNA: 3'- uaUGGGaCGGgUc-GGAGCaaCUCGCG- -5' |
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| 23248 | 5' | -58.1 | NC_005259.1 | + | 43900 | 0.66 | 0.610757 |
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Target: 5'- uUGCCC-GCgCCGGCUgaUCGaUGGGCaGCg -3' miRNA: 3'- uAUGGGaCG-GGUCGG--AGCaACUCG-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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