miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23271 3' -55.1 NC_005259.1 + 18861 0.66 0.789003
Target:  5'- cCGUGCaacgguuGCGCGCGUGGGAggccaaccacgaUGUcgACCUCGg -3'
miRNA:   3'- -GCACG-------CGCGUGUGUCCU------------ACA--UGGAGC- -5'
23271 3' -55.1 NC_005259.1 + 41682 0.68 0.676673
Target:  5'- gCGUGCguggccaccGCGCACACAGGcAUGcagccgccgcaUACCggCGg -3'
miRNA:   3'- -GCACG---------CGCGUGUGUCC-UAC-----------AUGGa-GC- -5'
23271 3' -55.1 NC_005259.1 + 24261 0.75 0.316282
Target:  5'- aGUGCGUGCACGCGuGGcUGgaagcgcucgACCUCGg -3'
miRNA:   3'- gCACGCGCGUGUGU-CCuACa---------UGGAGC- -5'
23271 3' -55.1 NC_005259.1 + 3194 0.77 0.228471
Target:  5'- gGUGCGCGCAC-CAcGAUGUgggGCCUCa -3'
miRNA:   3'- gCACGCGCGUGuGUcCUACA---UGGAGc -5'
23271 3' -55.1 NC_005259.1 + 65049 0.82 0.116903
Target:  5'- -cUGCGCGC-CACAGGuAUGUGCCUUGu -3'
miRNA:   3'- gcACGCGCGuGUGUCC-UACAUGGAGC- -5'
23271 3' -55.1 NC_005259.1 + 28407 0.85 0.06483
Target:  5'- gGUGCccGCGCGCAUGGGcAUGUACCUCGg -3'
miRNA:   3'- gCACG--CGCGUGUGUCC-UACAUGGAGC- -5'
23271 3' -55.1 NC_005259.1 + 29420 1.1 0.0013
Target:  5'- uCGUGCGCGCACACAGGAUGUACCUCGg -3'
miRNA:   3'- -GCACGCGCGUGUGUCCUACAUGGAGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.