Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23284 | 3' | -56.5 | NC_005259.1 | + | 8314 | 0.67 | 0.645005 |
Target: 5'- cCGCCuuGucGGAGCUgccgaCGGCGAUCAg -3' miRNA: 3'- -GUGGggCucUUUCGGa----GCCGCUAGUg -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 1292 | 0.68 | 0.601937 |
Target: 5'- gACCCCGAGGucGAGCuCUacGCGGUCGu -3' miRNA: 3'- gUGGGGCUCU--UUCG-GAgcCGCUAGUg -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 51779 | 0.68 | 0.601937 |
Target: 5'- gCGCCuuGAGGuauucGGCgCUCGGCgGGUCGg -3' miRNA: 3'- -GUGGggCUCUu----UCG-GAGCCG-CUAGUg -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 45378 | 0.67 | 0.612689 |
Target: 5'- uUugCCCGAGGAAuuUCUCGG-GGUCGCc -3' miRNA: 3'- -GugGGGCUCUUUc-GGAGCCgCUAGUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 29915 | 0.67 | 0.621302 |
Target: 5'- gCugCCCGAGAccaucgacgcguGGCCgccacgCGGCccGGUCGCg -3' miRNA: 3'- -GugGGGCUCUu-----------UCGGa-----GCCG--CUAGUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 49030 | 0.67 | 0.623457 |
Target: 5'- cCACCCCGGucGAGUCggcaCGGaCGAUgACg -3' miRNA: 3'- -GUGGGGCUcuUUCGGa---GCC-GCUAgUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 17152 | 0.67 | 0.633154 |
Target: 5'- gCACCCCGcagaagaAGAAAGCUgacgacaaGGCGG-CGCa -3' miRNA: 3'- -GUGGGGC-------UCUUUCGGag------CCGCUaGUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 33307 | 0.67 | 0.634232 |
Target: 5'- gCGgCUCGAucccGCCgUCGGCGGUCACg -3' miRNA: 3'- -GUgGGGCUcuuuCGG-AGCCGCUAGUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 60910 | 0.67 | 0.634232 |
Target: 5'- cCACCaucgugaaaCCGAuGAGcucGGCCUUGGUGAUCGu -3' miRNA: 3'- -GUGG---------GGCU-CUU---UCGGAGCCGCUAGUg -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 58853 | 0.67 | 0.638542 |
Target: 5'- uCACCCuCGGGcucgccgucgucgucGucGCCUCGGC-AUCGCc -3' miRNA: 3'- -GUGGG-GCUC---------------UuuCGGAGCCGcUAGUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 41263 | 0.68 | 0.59121 |
Target: 5'- cCGCCCCGGucGAGCacccucgUCGGCGcacucauucGUCACg -3' miRNA: 3'- -GUGGGGCUcuUUCGg------AGCCGC---------UAGUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 46519 | 0.67 | 0.645005 |
Target: 5'- gAUCCCGc--GAGCCggUCGGgGAUCGCc -3' miRNA: 3'- gUGGGGCucuUUCGG--AGCCgCUAGUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 64760 | 0.67 | 0.655768 |
Target: 5'- gCGCCUCGcGAcggugcGCCUCGGCGGccCGCu -3' miRNA: 3'- -GUGGGGCuCUuu----CGGAGCCGCUa-GUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 67374 | 0.66 | 0.677219 |
Target: 5'- gAUCuuGGGGAaauuacgcuuGGCCUCGGgGGugUCACg -3' miRNA: 3'- gUGGggCUCUU----------UCGGAGCCgCU--AGUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 22384 | 0.66 | 0.687888 |
Target: 5'- aAgCCCG-GucGGCCUCGGCGAc--- -3' miRNA: 3'- gUgGGGCuCuuUCGGAGCCGCUagug -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 13237 | 0.66 | 0.698507 |
Target: 5'- gGCCaCCGuGAGGGCCgagcaccCGGCGGUa-- -3' miRNA: 3'- gUGG-GGCuCUUUCGGa------GCCGCUAgug -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 44749 | 0.66 | 0.717456 |
Target: 5'- cCGCgCCGAGGcucuuGAGCaccgaucccgcaUCGGCGGUCGa -3' miRNA: 3'- -GUGgGGCUCU-----UUCGg-----------AGCCGCUAGUg -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 4720 | 0.66 | 0.718502 |
Target: 5'- cCGCgCCCGAGAAAGCCcgcaaggaccggcUCaGGcCGGUCc- -3' miRNA: 3'- -GUG-GGGCUCUUUCGG-------------AG-CC-GCUAGug -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 7707 | 0.66 | 0.719547 |
Target: 5'- cCACCaucgucugggCCGAGGGAuaccucacccGCCUCGaggacGCGGUCACc -3' miRNA: 3'- -GUGG----------GGCUCUUU----------CGGAGC-----CGCUAGUG- -5' |
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23284 | 3' | -56.5 | NC_005259.1 | + | 42649 | 0.66 | 0.729948 |
Target: 5'- gGCCCCGAugugGgcGGCCacgcggcugcugUUGGCGAUCuCg -3' miRNA: 3'- gUGGGGCU----CuuUCGG------------AGCCGCUAGuG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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