Results 1 - 20 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23286 | 5' | -61.7 | NC_005259.1 | + | 43240 | 0.66 | 0.459774 |
Target: 5'- -aGCGaGCUGacgauGCCgccCgCGCCGGGUGCc -3' miRNA: 3'- agUGCgCGAC-----CGGau-G-GCGGCCCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 50693 | 0.66 | 0.45044 |
Target: 5'- -gAgGCGCUGcucgucgucgcGCUUGa-GCCGGGUGCu -3' miRNA: 3'- agUgCGCGAC-----------CGGAUggCGGCCCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 48171 | 0.66 | 0.45044 |
Target: 5'- -aAC-CGCccccGCC-GCCGCCGGGUGUc -3' miRNA: 3'- agUGcGCGac--CGGaUGGCGGCCCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 41373 | 0.66 | 0.454161 |
Target: 5'- -aGCGUGCggucgagcuUGGCCgguacccauggcgcGCCGCUcgGGGUGCc -3' miRNA: 3'- agUGCGCG---------ACCGGa-------------UGGCGG--CCCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 45746 | 0.66 | 0.468262 |
Target: 5'- --cCGCGCaccGCCUcggggaugagcacGCCcucGCCGGGUGCc -3' miRNA: 3'- aguGCGCGac-CGGA-------------UGG---CGGCCCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 47862 | 0.66 | 0.45044 |
Target: 5'- -aGCGCcuuGUUGGCCUcuuccaGCUugGCCGGGUcaGCg -3' miRNA: 3'- agUGCG---CGACCGGA------UGG--CGGCCCA--CG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 9398 | 0.66 | 0.43209 |
Target: 5'- gCACGCGC--GCC--CCGguaCGGGUGCg -3' miRNA: 3'- aGUGCGCGacCGGauGGCg--GCCCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 21952 | 0.66 | 0.441211 |
Target: 5'- aCugGCGCaaGGCgUGacCCGCgUGGGUGUc -3' miRNA: 3'- aGugCGCGa-CCGgAU--GGCG-GCCCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 4426 | 0.66 | 0.459774 |
Target: 5'- cCGCGCGCccgaGGUCgaGCCGCCc-GUGCg -3' miRNA: 3'- aGUGCGCGa---CCGGa-UGGCGGccCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 40440 | 0.67 | 0.414184 |
Target: 5'- gUCugGUGUgUGGCCUGCCGgUGuGGUu- -3' miRNA: 3'- -AGugCGCG-ACCGGAUGGCgGC-CCAcg -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 56899 | 0.67 | 0.405404 |
Target: 5'- aUCGCaGCG-UGGCC-GCCGCagcaGGcGUGCc -3' miRNA: 3'- -AGUG-CGCgACCGGaUGGCGg---CC-CACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 33138 | 0.67 | 0.396742 |
Target: 5'- cCGCGCGUgaucuuGCCguagACCGCCugaggcaccgGGGUGUc -3' miRNA: 3'- aGUGCGCGac----CGGa---UGGCGG----------CCCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 37229 | 0.67 | 0.377278 |
Target: 5'- -gGgGCGCUGGCCcgugccggauuggcUGCCGCCGu-UGCc -3' miRNA: 3'- agUgCGCGACCGG--------------AUGGCGGCccACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 45410 | 0.67 | 0.371483 |
Target: 5'- -aGCGCGCugaUGGCC-GCCGCCGc-UGCc -3' miRNA: 3'- agUGCGCG---ACCGGaUGGCGGCccACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 46042 | 0.68 | 0.326592 |
Target: 5'- -uGCGUGaccucguagccgaUGGCCUugagcugcucaagcACCGCCGGG-GCg -3' miRNA: 3'- agUGCGCg------------ACCGGA--------------UGGCGGCCCaCG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 66254 | 0.68 | 0.331882 |
Target: 5'- aCGCaGCGC-GGCCcg-CGCCGGGcgGCg -3' miRNA: 3'- aGUG-CGCGaCCGGaugGCGGCCCa-CG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 47812 | 0.68 | 0.331882 |
Target: 5'- gUCGCGagaccuCGCgGGCCaccgcUGCCGCUGacGGUGCg -3' miRNA: 3'- -AGUGC------GCGaCCGG-----AUGGCGGC--CCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 45516 | 0.68 | 0.339548 |
Target: 5'- cCAcCGCGCcGGUgUugGCCGCCGuGGcUGCg -3' miRNA: 3'- aGU-GCGCGaCCGgA--UGGCGGC-CC-ACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 53718 | 0.68 | 0.355263 |
Target: 5'- gUCAcCGCGCcgcugaUGGUgaUGUCGCCGGGUGUc -3' miRNA: 3'- -AGU-GCGCG------ACCGg-AUGGCGGCCCACG- -5' |
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23286 | 5' | -61.7 | NC_005259.1 | + | 50021 | 0.68 | 0.366564 |
Target: 5'- aCGgGCGCUgcguaauccgcuguGGCCUcgacACCGCCccgcacgcgaaacgGGGUGUa -3' miRNA: 3'- aGUgCGCGA--------------CCGGA----UGGCGG--------------CCCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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