Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2329 | 5' | -58.8 | NC_001416.1 | + | 21117 | 0.67 | 0.401939 |
Target: 5'- -aGCcc-UGC-CAGCGCCGUCAGUGUc -3' miRNA: 3'- uaUGacuACGcGUCGCGGCGGUCGCG- -5' |
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2329 | 5' | -58.8 | NC_001416.1 | + | 35540 | 0.7 | 0.233203 |
Target: 5'- cUGCUGAUGUGCucAGUaucaCCGCCAGUGg -3' miRNA: 3'- uAUGACUACGCG--UCGc---GGCGGUCGCg -5' |
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2329 | 5' | -58.8 | NC_001416.1 | + | 40378 | 0.7 | 0.233203 |
Target: 5'- uUACUGuUGCGCcuguuAGCGCggcaaCGuCCGGCGCa -3' miRNA: 3'- uAUGACuACGCG-----UCGCG-----GC-GGUCGCG- -5' |
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2329 | 5' | -58.8 | NC_001416.1 | + | 46078 | 0.66 | 0.459655 |
Target: 5'- cAUGCaGAUGCGuCAGCGUgauguUGCU-GCGCu -3' miRNA: 3'- -UAUGaCUACGC-GUCGCG-----GCGGuCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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