Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23297 | 5' | -51.6 | NC_005259.1 | + | 30748 | 0.68 | 0.829751 |
Target: 5'- ----aGAGUUCAUgaacugcaugccgaCGCCGGUGGCACCg -3' miRNA: 3'- gcccaUUCAAGUA--------------GUGGUCGUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 62130 | 0.66 | 0.929181 |
Target: 5'- cCGaGGUGA--UCGUCucgACCAccucaggccGCAGCACCu -3' miRNA: 3'- -GC-CCAUUcaAGUAG---UGGU---------CGUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 26657 | 0.66 | 0.923366 |
Target: 5'- -cGGcAGGUUCGgcgCAcCCGGCGGCAgCCc -3' miRNA: 3'- gcCCaUUCAAGUa--GU-GGUCGUCGU-GG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 42061 | 0.66 | 0.917269 |
Target: 5'- gGGGUGGGcggCuaggUAUC-GCAGCACCg -3' miRNA: 3'- gCCCAUUCaa-Gua--GUGGuCGUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 65111 | 0.66 | 0.91089 |
Target: 5'- -cGGUcAGUgCGUCACCgucGGC-GCACCg -3' miRNA: 3'- gcCCAuUCAaGUAGUGG---UCGuCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 45760 | 0.67 | 0.904232 |
Target: 5'- gGGGaUGAGcaCGcccUCGCCGGgugcCAGCACCg -3' miRNA: 3'- gCCC-AUUCaaGU---AGUGGUC----GUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 8553 | 0.67 | 0.904232 |
Target: 5'- gCGGuGUAGcc-CAUgCGCC-GCAGCACCg -3' miRNA: 3'- -GCC-CAUUcaaGUA-GUGGuCGUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 51344 | 0.67 | 0.890089 |
Target: 5'- -cGGUGAGcUCGgauaugC-CCGGUGGCACCg -3' miRNA: 3'- gcCCAUUCaAGUa-----GuGGUCGUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 29195 | 0.67 | 0.882613 |
Target: 5'- uGGGgcggauGGUUCGcccccgcaugguUCGCCAGCcucGGCGCg -3' miRNA: 3'- gCCCau----UCAAGU------------AGUGGUCG---UCGUGg -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 66940 | 0.67 | 0.881851 |
Target: 5'- gGGGUGAGcgggaCACCGGCGagcuguuucuugcGCGCCu -3' miRNA: 3'- gCCCAUUCaaguaGUGGUCGU-------------CGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 58694 | 0.67 | 0.874874 |
Target: 5'- gGGGUcGGcUCGUCGCCGccGCcGuCACCa -3' miRNA: 3'- gCCCAuUCaAGUAGUGGU--CGuC-GUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 49558 | 0.68 | 0.850145 |
Target: 5'- cCGGGcu-GUUCGUCggucguggccagACCGGCgggcggggucAGCACCu -3' miRNA: 3'- -GCCCauuCAAGUAG------------UGGUCG----------UCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 57271 | 0.68 | 0.850145 |
Target: 5'- uGGGcGAGggauUgACCGGUGGCACCu -3' miRNA: 3'- gCCCaUUCaaguAgUGGUCGUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 46400 | 0.68 | 0.832477 |
Target: 5'- gCGcGGUGAGggCAUCgaaACCGgGCuguGCACCc -3' miRNA: 3'- -GC-CCAUUCaaGUAG---UGGU-CGu--CGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 702 | 0.68 | 0.83157 |
Target: 5'- gGGGUcugaacaugAGGUggcaucgcgagaaUCA-CACCGGCGGUGCCu -3' miRNA: 3'- gCCCA---------UUCA-------------AGUaGUGGUCGUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 19765 | 1.14 | 0.001503 |
Target: 5'- gCGGGUAAGUUCAUCACCAGCAGCACCa -3' miRNA: 3'- -GCCCAUUCAAGUAGUGGUCGUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 28743 | 0.69 | 0.817719 |
Target: 5'- aGGGUuacgcgcccgagcguGAGaUCGUCGCCGGUgccgacgaGGUACCc -3' miRNA: 3'- gCCCA---------------UUCaAGUAGUGGUCG--------UCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 21975 | 0.69 | 0.813948 |
Target: 5'- uGGGUGucGUUC-UCcCCAcCGGCACCg -3' miRNA: 3'- gCCCAUu-CAAGuAGuGGUcGUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 21759 | 0.69 | 0.804385 |
Target: 5'- uCGGGUAAGUUCugauggACCGGCuG-ACCu -3' miRNA: 3'- -GCCCAUUCAAGuag---UGGUCGuCgUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 49612 | 0.69 | 0.798558 |
Target: 5'- uCGGGcagcucaucaUCGUCAUCGGCGGCAgCCg -3' miRNA: 3'- -GCCCauuca-----AGUAGUGGUCGUCGU-GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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