Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23297 | 5' | -51.6 | NC_005259.1 | + | 53506 | 0.7 | 0.764401 |
Target: 5'- -cGGUGAacaacGUgcUCGUgGCCAGCGGCACg -3' miRNA: 3'- gcCCAUU-----CA--AGUAgUGGUCGUCGUGg -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 55 | 0.7 | 0.764401 |
Target: 5'- uCGGGUAacuuAGUUCGUguCaGGCGGgACCg -3' miRNA: 3'- -GCCCAU----UCAAGUAguGgUCGUCgUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 60597 | 0.7 | 0.732915 |
Target: 5'- uCGGGUGc---CGUCAUCgAGCAGCACa -3' miRNA: 3'- -GCCCAUucaaGUAGUGG-UCGUCGUGg -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 62363 | 0.71 | 0.700498 |
Target: 5'- cCGGGUAGGaUCAgcUCAUCgAGUAGCAgauCCa -3' miRNA: 3'- -GCCCAUUCaAGU--AGUGG-UCGUCGU---GG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 19619 | 0.71 | 0.671896 |
Target: 5'- uGGGUcugcGGUUCAaccucauugagacgcUCACCGGCAcCGCCu -3' miRNA: 3'- gCCCAu---UCAAGU---------------AGUGGUCGUcGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 26032 | 0.71 | 0.66636 |
Target: 5'- gCGGGU-------UgGCCAGCAGCGCCg -3' miRNA: 3'- -GCCCAuucaaguAgUGGUCGUCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 31615 | 0.72 | 0.656376 |
Target: 5'- uCGGGUcu-UUUGUCGCCAGCGcguuguacuGCGCCa -3' miRNA: 3'- -GCCCAuucAAGUAGUGGUCGU---------CGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 13796 | 0.73 | 0.578662 |
Target: 5'- aGuGGUGcga-CAUCACCGGCgAGCACCa -3' miRNA: 3'- gC-CCAUucaaGUAGUGGUCG-UCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 22075 | 0.74 | 0.513653 |
Target: 5'- cCGuGGUGAGcUCAUCgACCAGauggcgGGCACCa -3' miRNA: 3'- -GC-CCAUUCaAGUAG-UGGUCg-----UCGUGG- -5' |
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23297 | 5' | -51.6 | NC_005259.1 | + | 19765 | 1.14 | 0.001503 |
Target: 5'- gCGGGUAAGUUCAUCACCAGCAGCACCa -3' miRNA: 3'- -GCCCAUUCAAGUAGUGGUCGUCGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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