Results 61 - 68 of 68 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23298 | 5' | -53.1 | NC_005259.1 | + | 5974 | 0.73 | 0.458033 |
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Target: 5'- -cGACCACCAacuacacgGGCAGccGAGAACGCUc- -3' miRNA: 3'- uaCUGGUGGU--------UCGUC--UUCUUGCGGcu -5' |
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| 23298 | 5' | -53.1 | NC_005259.1 | + | 16568 | 0.74 | 0.399939 |
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Target: 5'- -cGcCCACCGGGCAGAu---CGCCGAg -3' miRNA: 3'- uaCuGGUGGUUCGUCUucuuGCGGCU- -5' |
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| 23298 | 5' | -53.1 | NC_005259.1 | + | 11017 | 0.74 | 0.390722 |
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Target: 5'- -gGACgCGCCgAGGCAGGAGuuCGCCGGg -3' miRNA: 3'- uaCUG-GUGG-UUCGUCUUCuuGCGGCU- -5' |
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| 23298 | 5' | -53.1 | NC_005259.1 | + | 13536 | 0.75 | 0.338414 |
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Target: 5'- -cGGCCACCAAGCAGuucgcgauguGGccCGCCGAc -3' miRNA: 3'- uaCUGGUGGUUCGUCu---------UCuuGCGGCU- -5' |
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| 23298 | 5' | -53.1 | NC_005259.1 | + | 58423 | 0.75 | 0.338414 |
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Target: 5'- -aGAuCCACCGGGCAGAuu-GCGCUGAu -3' miRNA: 3'- uaCU-GGUGGUUCGUCUucuUGCGGCU- -5' |
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| 23298 | 5' | -53.1 | NC_005259.1 | + | 16707 | 0.77 | 0.291384 |
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Target: 5'- gGUGACCGCCGAGCAGGuc-GCGCUc- -3' miRNA: 3'- -UACUGGUGGUUCGUCUucuUGCGGcu -5' |
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| 23298 | 5' | -53.1 | NC_005259.1 | + | 30547 | 0.78 | 0.249626 |
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Target: 5'- cAUGGCCACCGAGCGGuuGuuGACGCCc- -3' miRNA: 3'- -UACUGGUGGUUCGUCuuC--UUGCGGcu -5' |
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| 23298 | 5' | -53.1 | NC_005259.1 | + | 19284 | 1.07 | 0.00239 |
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Target: 5'- cAUGACCACCAAGCAGAAGAACGCCGAg -3' miRNA: 3'- -UACUGGUGGUUCGUCUUCUUGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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